大白菜—结球甘蓝1号染色体二体异附加系的获得与鉴定  

Generation and Identification of Chinese Cabbage–Cabbage Disomic Alien Addition Line 1

作　　者：吕文欣[1] 王彦华[1] 赵建军[1] 顾爱侠[1] 李艳霞[1] 管志坤[1] 闻晓英[1] 申书兴[1] 

机构地区：[1]河北农业大学园艺学院,河北保定071000

出　　处：《园艺学报》2011年第7期1275-1282,共8页Acta Horticulturae Sinica

基　　金：国家自然科学基金项目(30871713);河北省自然科学基金项目(C2010000677)

摘　　要：以大白菜附加结球甘蓝1号和6号染色体的双单体异附加系为材料,从其自交后代38个植株中筛选出3株2n=22的植株。通过核型分析及SSR鉴定确定这3个植株为大白菜—结球甘蓝1号染色体二体异附加系,其外观性状在营养生长时期与二倍体大白菜没有明显的差异,在生殖生长期具有部分结球甘蓝的特征,其减数分裂终变期染色体联会时,11个二价体的比率达90.10%,后期Ⅱ以11-11-11-11分离的比例为83.50%,从而证明了具有很高的遗传稳定性。The double monosomic addition lines of Chinese cabbage with No.1 and 6 chromosomes from cabbage were used for generating Chinese cabbage–cabbage disomic alien addition line.The results showed as follows：（1）Three 2n = 22 plants were screened from the 38 plants of the double monosomic addition lines selfing progeny.（2）Through karyotypic analysis and SSR identification,the three 2n = 22 plants were assigned as Chinese cabbage disomic addition line with No.1 chromosome from cabbage.（3） In the vegetative growth period,there were no significant differences between the disomic addition line and diploid Chinese cabbage,however they had some characteristics of cabbage in reproductive growth stage.（4）The observation of meiotic behavior showed that the rate of 11 bivalents was 90.10% at diakinesis,and the rate of chromosome distribution with 11-11-11-11 was 83.50% at anaphaseⅡ,which proved that the disomic addition line have a high genetic stability.

关 键 词：大白菜 结球甘蓝 二体异附加系 减数分裂 SSR 

分 类 号：S634.1[农业科学—蔬菜学]