β-连环蛋白和细胞周期蛋白D1在人乳头瘤病毒感染的子宫颈病变中的表达及临床病理意义  

Expression and clinicopathological significance of β-catenin and Cyclin D1 in cervical lesions infected with human papilloma virus

作　　者：蒋欣[1] 孙卫华[2] 王刚[3] 王彦青[1] 张亚军[1] 程学亮[1] 

机构地区：[1]江苏省连云港市妇幼保健院病理科,江苏连云港222006 [2]江苏省连云港市妇幼保健院妇产科,江苏连云港222006 [3]江苏省连云港市妇幼保健院检验科,江苏连云港222006

出　　处：《诊断学理论与实践》2011年第3期273-277,共5页Journal of Diagnostics Concepts & Practice

基　　金：连云港市卫生局科研资助项目(1023)

摘　　要：目的:探讨β-连环蛋白(β-catenin)和细胞周期蛋白D1(CyclinD1)在人乳头瘤病毒(HPV)感染的子宫颈病变中的表达及临床病理意义。方法:采用免疫组化法和HybriMax导流杂交基因芯片技术,检测100例HPV感染的不同类型子宫颈病变组织中β-catenin和CyclinD1蛋白的表达,并以20例正常子宫颈组织作为正常对照组进行分析。结果:β-catenin在子宫颈鳞状细胞癌(CSCC)组中的异常表达率较低级别子宫颈上皮内瘤变(CIN-L)组及正常对照组有显著增高(P均<0.05);同时,高级别子宫颈上皮内瘤变(CIN-H)组的β-catenin异常表达率亦较CIN-L组及正常对照组有显著增高(P均<0.05);而CIN-L组与正常组间比较,CSCC组与CIN-H组间比较,β-catenin异常表达率差异均无统计学意义(P>0.05)。随着子宫颈病变严重程度的增加,CyclinD1的阳性表达率及染色强度呈递增趋势,在CIN和CSCC组织的细胞质及细胞核中β-catenin异常表达与CyclinD1表达呈正相关。结伦:β-catenin及CyclinD1蛋白表达异常与HPV感染的子宫颈病变密切相关。Objective To investigate the expression and the clinicopathological significance of β-catenin and CyclinD1 in cervical lesions infected with human papilloma virus（HPV）.Methods Immunohistochemistry and HybriMax gene arrays and flow-through hybridization were used to detect the expressions of β-catenin and CyclinD1 in 100 cervical lesions of various diseases infected with HPV,and 20 normal cervical tissue samples were served as controls.Results The abnormal expression rate of β-catenin in cervical squamous cell cancer（CSCC） group was significantly higher than that in low grade cervical intraepithelial neoplasia（CIN-L） group and normal control group（P0.05）.The abnormal expression rate of β-catenin in high grade cervical intraepithelial neoplasia（CIN-H） group was significantly higher than that in CIN-L group and normal control group（P0.05）.However,there was no significant difference between CIN-L group and normal control group,CSCC group and CIN-H group（P0.05）.The positive expression rate and staining intensity of CyclinD1 increased with the increase in disease severity of cervical lesion.In both CSCC group and CIN group,the abnormal expression of β-catenin correlated positively with expression of CyclinD1.Conclusions There is a close correlation of abnormal expression of β-catenin and CyclinD1 with HPV infected cervical lesions.

关 键 词：子宫颈上皮内瘤变 Β-连环蛋白 细胞周期蛋白D1 人乳头瘤病毒 

分 类 号：R711.74[医药卫生—妇产科学]