CD4^+稳定自然杀伤T细胞在实验性变应性鼻炎发病中的作用  

Role of CD4^+ invariant natural killer T cells in experimental allergic rhinitis

作　　者：王凯[1] 郑春泉[1,2] 

机构地区：[1]复旦大学附属眼耳鼻喉科医院耳鼻喉科,上海200031 [2]江苏省常州市第一人民医院耳鼻喉科,常州213003

出　　处：《中国眼耳鼻喉科杂志》2011年第4期208-210,273,共4页Chinese Journal of Ophthalmology and Otorhinolaryngology

摘　　要：目的探讨实验性变应性鼻炎发病过程中CD4+稳定自然杀伤T(CD4+iNKT)细胞的浸润情况。方法将30只C57BL/6小鼠随机分为变应性鼻炎组(A组)和健康对组照(B照)。比较两组小鼠的变应性鼻炎症状评分、鼻黏膜嗜酸性粒细胞(EOS)计数、鼻黏膜组织形态;采用免疫酶标技术检测两组小鼠鼻黏膜CD4+iNKT细胞的浸润情况;采用流式细胞技术(FCM)检测鼻腔黏膜中的CD4+iNKT细胞。运用SPSS11.5统计学软件进行分析。结果①A组小鼠症状评分为(7.3±1.0)分,较B组的(0.7±0.6)分明显增高(P<0.01)。②A组EOS计数为(7.5±1.2)个/HP,较B组的(0.4±0.5)个/HP明显增高(P<0.01)。③苏木素-伊红染色可见:A组纤毛结构大量脱落、组织水肿、小血管增生、黏膜上皮下固有层EOS及淋巴细胞浸润;B组无明显EOS浸润,纤毛结构完整。④A组鼻黏膜下有多量CD4+iNKT细胞;B组仅见少量CD4+细胞。⑤A组鼻黏膜CD4+iNKT细胞阳性率为72.6%±7.9%,明显高于B组的0.49%±0.1%(P<0.01)。结论变应性鼻炎小鼠鼻腔黏膜有大量的CD4+iNKT细胞浸润,数量远远超过CD4+T细胞。Objective To observe the infiltration of invariant natural killer T（iNKT） cells in the nasal mucosa of mice with allergic rhinitis. Methods Thirty C57BL/6 mice were randomly divided into allergic rhinitis group （group A） and control group （group B）. The symptom score, eosinophils（EOS） count and histological morphous were compared between the two groups. The expression of CD4 NK1.1 was detected by immunoenzymatic technique. The positive rate of the iNKT cells in CD4 ＋ cells was determined by flow cytometry （FCM）. The data were analyzed by SPSS 11.5 statistics software. Results The score in group A（7.3 ± 1.0） was higher than that in group B（0.7 ± 0.6） （P 〈 0.01 ）. The EOS count in the epithelium in group A（7.5 ± 1.2） per HP was more than that in group B （0.4 ± 0.5 ） per HP （P 〈 0.01 ）. Histological morphous staining showed that there were cilia defluxion, tissue dropsy, vascular proliferation and EOS infiltration in group A but non in group B. The expression of enhanced fluorescence proteins corresponding to CD4 NK1.1 showed that there were many iNKT cells in the mucosa in group A and only several CD4 ＋ T cells in group B. FCM showed that the positive rate of iNKT cells in group A （72.6% ± 7.9% ） was higher than that in group B （0.49% ± 0.1% ） （P 〈 0.01 ）. Conclusions CD4 ＋ iNKT cells played a prominent pathogenic role in experimental allergic rhinitis.

关 键 词：鼻炎 变应性 免疫球蛋白E 自然杀伤T细胞 

分 类 号：R765.21[医药卫生—耳鼻咽喉科]