姜黄素对LPS诱导小鼠caeVEGF表达的抑制  

Inhibition of curcumin on LPS-induced caeVEGF in mice

作　　者：甘力[1] 蒋福升[1] 范秋艳[1] 周洋[1] 林瑶瑶[1] 阮润[1] 丁志山[1] 

机构地区：[1]浙江中医药大学生命科学学院,浙江杭州310053

出　　处：《中国药理学通报》2011年第8期1148-1151,共4页Chinese Pharmacological Bulletin

基　　金：浙江重点科技创新团队资助项目(No2011R09042-04);浙江省中医药重点项目研究计划资助项目(No2005Z001);浙江中医药大学科研基金资助项目(No17110024)

摘　　要：目的通过小鼠血管内皮生长因子(VEGF)上游调控序列caeVEGF的作用,探索在LPS诱导下姜黄素抑制表达的情况。方法构建caeVEGF-EGFP真核表达重组质粒,通过转染HeLa细胞,获得稳定转染细胞株。采用LPS诱导,结合阳性药物姜黄素处理,利用荧光显微镜及流式细胞仪观察、分析GFP荧光表达量。结果所建立的稳定转染细胞株在荧光显微镜下可见微弱荧光,经100μg.L-1 LPS诱导后,荧光强度增强,约为诱导前的4.3倍;而阳性药物姜黄素可以剂量依赖性抑制LPS诱导的荧光增强,其中6μmol.L-1处理24 h抑制率达56.5%。结论该研究所建立的抗血管生成筛药平台是可行的,这为进一步高通量筛选抗血管生成药物奠定了良好基础。Aim To study the inhibition of curcumin on LPS-induced caeVEGF in mice.Methods caeVEGF-EGFP was constructed and a cell line with stable expression of caeVEGF-EGFP was established by gene transfection.The probability of the cell line as an antiangiogenic drug screening model was verified.Fluorescense microscopy and flow cytometry method were carried out to evaluate the expression of GFP after treated by LPS and series concentration of curcumin.Results The cell line with hypofluorescence was observed,which was dramatically increased to 4.3 folds after treated by 100 μg·L-1 LPS.However,curcumin co-treatment with LPS could dose-dependently lower the expression of GFP,and 56.5% was inhibited when treated with 6 μmol·L-1 curcumin for 24h.Conclusion The results above confirmsd the feasibility of our idea,and the work paves the way for further high through screening antiangiogenic drug.

关 键 词：血管内皮生长因子 绿色荧光蛋白 HELA细胞 抗血管生成 姜黄素 LPS 

分 类 号：R332[医药卫生—人体生理学] R282.71[医药卫生—基础医学]