补阳还五汤提高血管性痴呆大鼠海马CREB、C/EBP的DNA结合活性  

Effect of Buyanghuanwutang on the improvement of the DNA-binding activity of CREB and C/EBP in hippocampus of vascular dementia rats

作　　者：张泓波[1] 高维娟[2] 钱涛[3] 唐敬龙[1] 李君[1] 

机构地区：[1]承德医学院病理生理学教研室,河北承德067000 [2]河北化工医药职业技术学院,河北石家庄050026 [3]河北省人民医院,河北石家庄050051

出　　处：《中国药理学通报》2011年第8期1166-1170,共5页Chinese Pharmacological Bulletin

基　　金：河北省人才工程培养项目(No2010353);河北省卫生厅医学科研重点课题(No20090582)

摘　　要：目的观察补阳还五汤对血管性痴呆大鼠海马CREB(cAMP反应元件结合蛋白)、C/EBP(CCAAT/增强子结合蛋白)DNA结合活性的影响。方法实验分为假手术组、模型组、补阳还五汤组和尼莫地平组。采用改良的4血管法制备血管性痴呆模型,分别给予生理盐水、补阳还五汤、尼莫地平灌胃干预,用凝胶电泳迁移率改变分析法(EMSA)测定各组大鼠CREB和C/EBP的DNA结合活性。结果与假手术组比较,模型组大鼠海马组织CREB的DNA结合活性明显降低(P<0.01);与模型组比较,尼莫地平组与补阳还五汤组明显升高(P<0.01);尼莫地平组与补阳还五汤组之间差异无显著性(P>0.05)。结论补阳还五汤可提高VD大鼠海马组织CREB、C/EBP与DNA的结合活性。Aim To observe the influence of Buyanghuanwutang（BYHWT） on the DNA-binding activity of cAMP response element binding protein（CREB） and CCAAT/enhancer binding protein（C/EBP） in hippocampus of vascular dementia（VD） rats.Methods The rats were randomly divided into sham-operation group,VD model group,BYHWT-treated group and nimodipine-treated group.VD rat models were prepared by four-vessel occlusion,and physiological saline solution,BYHWT and nimodipine were administered respectively to four groups by gavage.Electrophoretic Mobility Shift Assay（EMSA） was used to observe the change of DNA-binding activity of CREB and C/EBP.Results The DNA-binding activity of CREB of rat hippocampus was significantly lower in VD model group than that in Sham-operation group（P0.01）.Compared with VD model group,both nimodipine-treated group and BYHWT-treated group were definitely higher（P0.01）.There was no significant difference between BYHWT-treated group and nimodipine-treated group（P0.05）.Conclusion BYHWT can improve the DNA-binding activity of CREB and C/EBP in hippocampus of VD rat.

关 键 词：补阳还五汤 血管性痴呆 海马组织 CAMP反应元件结合蛋白 CCAAT/增强子结合蛋白 DNA结合活性 

分 类 号：R332[医药卫生—人体生理学] R289.5[医药卫生—基础医学]