大鼠脂肪源性干细胞成脂诱导分化的实验研究  

Research of Rat's Adipose-Derived Stem Cells Induced Differentiation into Adipogenic Cells

作　　者：周明辉[1] 张晓薇[1] 李莉[1] 

机构地区：[1]广州医学院第一附属医院,广东广州510120

出　　处：《实用妇产科杂志》2011年第7期515-518,共4页Journal of Practical Obstetrics and Gynecology

摘　　要：目的:观察大鼠脂肪源性干细胞(ADSCs)向成脂诱导分化的生物学特性,以探讨ADSCs在治疗盆腔脏器脱垂(POP)中生物工程学的意义。方法:酶消化法体外分离培养大鼠ADSCs;相差显微镜观察ADSCs的细胞形态;运用流式细胞术鉴定细胞周期以及细胞表面抗原的表达;对ADSCs进行成脂诱导分化,油红"O"染色观察其向脂肪细胞分化的能力。结果:体外培养的ADSCs增殖迅速,呈成纤维细胞样生长,细胞呈多角形、长梭形,形态均一;细胞周期结果显示G0/G1期细胞占90.60%;表面抗原阳性表达率分别为CD44 99.88%,CD90 99.74%,CD105 95.00%,CD49d 89.56%,CD34 3.04%,CD106 0.85%,CD45 0.26%;成脂诱导实验细胞内出现脂滴,诱导14天后油红"O"染色呈橘红色。结论:ADSCs易于分离培养,具有向成脂诱导分化的功能,提示ADSCs可作为治疗POP的种子细胞。Objective：To observe the biological characteristics of the adipose-derived stem cells（ADSCs） differentiated into adipogenic cells,to investigate the feasibility of ADSCs as the seed cells in treatment of pelvic organ prolapse（POP）.Methods：ADSCs were isolated and cultured in vitro,and the morphological changes were observed by phase-contrast microscope.The expressions of surface antigenes and the cell cycle were detected by flow cytometry.After induced by adipogenic inducing factor,the differentiations to adipocytes were verified by oil red ＂O＂ staining.Results：ADSCs propagated rapidly in vitro,and constituted a homogenous fibroblast-like morphology,the shapes of cells were polygonal,and spindle;90.60% ADSCs were G0/G1 phase cells;the positive rates of cellular surface antigenes were CD44 99.88%,CD105 95.00%,CD90 99.74%,CD49d 89.56%,CD34 3.04%,CD106 0.85%,CD45 0.26%;after induction,intracellular lipid droplets could be observed and present jacinth by Oil Red stain in 14 days.Conclusions：ADSCs are easily isolated and cultured in vitro,and have the potential to differentiate into adipogenic cells.ADSCs can be as the seed cells for the treatment of POP.

关 键 词：组织工程 脂肪源性干细胞 诱导分化 

分 类 号：R711.4[医药卫生—妇产科学]