缺血再灌注损伤诱导心肌细胞自噬相关基因过表达  

Ischemia-reperfusion injury induced overexpression of autophagy-related genes in cardiomyocytes

作　　者：肖健[1] 何斌[2] 朱晓燕[3] 张宇峰[1] 康波[1] 王志农[1] 

机构地区：[1]第二军医大学长征医院胸心外科,上海200003 [2]上海交通大学附属新华医院麻醉科,上海200092 [3]第二军医大学基础部生理学教研室,上海200433

出　　处：《第二军医大学学报》2011年第7期776-778,共3页Academic Journal of Second Military Medical University

基　　金：国家自然科学基金(30901470)~~

摘　　要：目的探讨缺血再灌注(IR)损伤对心肌细胞自噬相关基因(autophagy-related gene,Atg)表达的影响。方法成年雄性SD大鼠随机分为2组:对照组(假手术组),于左前降支(left anterior descending,LAD)下方穿过5-0丝线,不结扎;缺血再灌注损伤组:结扎LAD,45 min后松结扎线再灌注120 min。利用RT-PCR方法,检测各组心肌Beclin 1和Atg5mRNA表达;应用蛋白质印迹分析法检测心肌LC3蛋白表达变化。结果缺血再灌注损伤后心肌Beclin 1和Atg5 mRNA表达较对照组升高,分别达到对照组的2.45和1.6倍,差异具有统计学意义(P<0.05);LC3-Ⅱ蛋白表达增加,IR后LC3-Ⅱ/LC3-Ⅰ比值较对照组升高(1.4 vs 0.2,P<0.01)。结论缺血再灌注损伤可以诱导心肌细胞Atg家族表达增加,促进自噬的发生,导致心肌损害。Objective To investigate the effect of ischemia-reperfusion（IR） injury on the expression of autophagy-related gene in cardiomyocytes.Methods Male adult SD rats,weighing 250-300 g,were randomly divided into 2 groups,namely,the control group,in which rats underwent thoracotomy without left anterior descending coronary（LAD） ligation,and IR group,in which the left anterior descending coronary arteries were ligated for 45 min,followed by reperfusion for 2 h.Beclin 1 and Atg5 mRNA expression levels were examined by RT-PCR in the myocardium,and the LC3 protein was determined by Western blotting analysis.Results Beclin 1 and Atg5 mRNA expression levels in IR group increased to 2.45 and 1.6 times those of the control group,respectively（P0.05）.The expression of LC3-Ⅱprotein was increased,which led to significant increase of LC3-Ⅱ/LC3-Ⅰratio in IR group（1.4 vs 0.2,P0.01）.Conclusion Ischemia-reperfusion injury can induce expression of Atg family,promoting autophagy and causing cardiomyocyte damage.

关 键 词：心肌再灌注损伤 心肌细胞 自噬 

分 类 号：R654.2[医药卫生—外科学]