人类胚胎冻存技术的初步研究  

PRIMARY RESEARCH ON THE HUMAN EMBRYO CRYOPRESERVATION

作　　者：李媛[1] 陈子江[1] 冯云[2] 高士友[2] 

机构地区：[1]山东医科大学 [2]山东省立医院生殖医学中心

出　　处：《山东医科大学学报》1999年第4期308-310,共3页Acta Academiae Medicinae Shandong

基　　金：国家自然科学基金!资助课题(39300136)

摘　　要：研究了不同冷冻与复苏方法以及不同移植周期法对人类胚胎冻存结果的影响。共复苏了１４９ 个胚胎，８９ 个（５９．７％ ）得以存活。在２０ 个移植周期中，５ 例（２５．０％ ）获得临床妊娠，２ 例已经分娩健康的婴儿。结果表明，采用缓慢降温至－ １２０℃再浸入液氮中冷冻及快速升温、逐步置换冷冻保护剂的方法复苏，胚胎的存活率（７２．７％ ）较高。The technique of human embryo preservation is a kind of assisted reproductive technology to preserve the residual embryos in the treatment cycle of in vitro fertilization and embryo transfer(IVF ET).Multiple factors can affect the survival rate of embryo cryopreservation.The effect of different cryopreserve protocol and transfer cycles on the results of embryo cryopreservation was studied.The authors have thawed 149 embryos,among these,89 embryos(59.7%)survived.Of the 20 transfer cycles, 5 patients got clinical pregnancy(25%),2 patients already had live and health babies.The method of cooling to 120℃ slowly,then emerging into liquid nitrogen,and thawing quickly,replacing the cryoprotectant stepwise is the best way to preserve embryos and will result in high survival rate(72.7%).But the influence of different transfer cycles is not prominent on the clinical pregnant rate of cryopreserved mbryos.

关 键 词：胚胎移植 冷冻保存 存活率分析 胚胎 

分 类 号：R321－33[医药卫生—人体解剖和组织胚胎学]