中药异位宁对实验性大鼠异位子宫内膜COX-2基因表达的影响  

Effects of YiWeiNing Recipe on Expressions of Cyclo-oxygenase-2 mRNA in Ectopic Endometrium of Rats with Endometriosis

作　　者：徐晓宇[1] 丛慧芳[1] 马文光[2] 马宝璋[2] 

机构地区：[1]黑龙江中医药大学附属二院妇科,黑龙江哈尔滨150001 [2]黑龙江中医药大学附属一院妇科,黑龙江哈尔滨150040

出　　处：《四川中医》2011年第7期28-30,共3页Journal of Sichuan of Traditional Chinese Medicine

摘　　要：目的:探讨环氧合酶-2(COX-2)在子宫内膜异位症(EMT)发病中的作用以及异位宁对其基因表达的影响。方法:采用自体子宫组织块移植法建立子宫内膜异位症大鼠模型。应用免疫组化及原位杂交的方法,检测异位子宫内膜中COX-2的表达。结果:模型组EMT大鼠异位内膜中COX-2基因表达增高明显,与正常对照组比较具有显著性差异(P<0.01);中药高剂量组COX-2基因表达明显减弱,与模型组比较,有显著性差异(P<0.05、P<0.01)。结论:中药异位宁具有抑制COX-2阳性表达,从而抑制异位内膜组织新生血管形成,达到治疗EMT的作用。Objective：To explore the functions of cyclooxygenase-2（COX-2）in rats with endometriosis（EMT）and the results of YiWeiNing Recipe（YWNR）,a compound Chinese herbal medicine,affecting gene expression of cyclooxygenase-2（COX-2）.Methods：Operational self-transplantation was applied in establishing the rat models.Detection of cyclooxygenase-2（COX-2）was conducted with hybridization in situ and LSAB.Results：The levels of cyclooxygenase-2 were markedly increased in model groups.There were significant differences in the expressions of COX-2 between high-dose YWNR treated group and untreated group.YWNR could reduce the level of COX-2.Conclusion：YWNR can treat EMT through reducing the positive expressions of COX-2 and inhibiting Angiogenesis in EMT.

关 键 词：异位宁 子宫内膜异位症 环氧合酶-2大鼠 

分 类 号：R285.5[医药卫生—中药学]