兔耳大神经端侧吻合后神经元胞体和神经干内cAMP的水平  

cAMP level in neuron pericaryon and nerve trunk of rabbit greater auricular nerve after end to side neurorrhaphy

作　　者：曹祖峰[1] 陈绍宗[1] 张盈华[1] 李跃军[1] 吕小星[1] 

机构地区：[1]第四军医大学唐都医院,陕西西安710038

出　　处：《第四军医大学学报》1999年第11期969-971,共3页Journal of the Fourth Military Medical University

摘　　要：目的：观察神经端侧吻合后供神经的神经节和神经干内ｃＡＭＰ水平变化，为探索神经再生机制提供实验依据．方法：新西兰兔５２只，随机分为Ａ，Ｂ，Ｃ组，Ａ组为两耳大神经端侧吻合实验组，Ｂ组为未吻合对照组，Ｃ组为正常耳大神经对照组，Ａ，Ｂ２组各按术后２，４，７，１４，３０，６０ｄ分为６个观察组，每组动物４只．观察到期后，用放射免疫分析技术检测供神经节、神经干和吻合口部位的ｃＡＭＰ含量．结果：在正常耳大神经的神经节、神经干和相当于吻合口部位标本中，ｃＡＭＰ含量分别为（１．６２±０．１２），（１．５５±０．１７）和（１．５５±０．１７）ｎｍｏｌ／ｇ；Ａ，Ｂ２组在以上３个部位的ｃＡＭＰ含量呈现相同的变化趋势，即术后早期（２ｄ）下降到正常水平以下，４ｄ陡然上升，７ｄ达到峰值，分别在６０ｄ和３０ｄ缓慢下降至正常．Ａ组的ｃＡＭＰ含量在术后４ｄ～３０ｄ各时间点均显著高于Ｂ组．结论：兔耳大神经端侧吻合后，供神经的神经节和神经干内ｃＡＭＰ含量显著高于不作神经端侧吻合的对照组，恢复到正常水平的时间也明显延迟．AIM: By observing the variation of cAMP in donor nerve neuron pericaryon and nerve trunk, to provide experimental imformation in exploring mechanism of nerve regeneration. METHODS: Fifty two New Zealand rabbits were randomly divided into three groups A,B,C. Double side greater auricular nerve in Group A was neurorrhaphied while in group B was unneurorrhaphied. Both group A and B were divided into six subgroups on the basis of postoperative time: 2, 4, 7, 14, 30, 60 d. Group C was normal greater auricular nerve and had no subgroup. cAMP level in donor nerve ganglion, nerve trunk and anastomosed position was determined by using radioimmunoassay at each time point. RESULTS:cAMP content in normal nerve ganglion, nerve trunk and anastomosed position was 1.62±0.12,1.55±0.17 and 1.55± 0.17 nmol/g respectively. The same variation pattern of cAMP level in the position of group A and B was occurred. cAMP level was lower than normal level in early time ( 2 d ) after operation. It rose rapidly on the 4th day and reached climax on the 7th day after operation. Then it fell gradually and restored to normal level on the 60th and 30th day respectively. cAMP level at each point time from 4~30 days of group A was higher than that of group B. CONCLUSION: When rabbit greater auricular nerve was end to side anastomosed, cAMP level in donor nerve ganglion and nerve trunk was significantly higher than that of operation control and restored to the normal level apparently slowly.

关 键 词：神经端侧吻合 腺苷环-磷酸 神经节 轴突 

分 类 号：R622.3[医药卫生—整形外科]