抑制和兴奋性氨基酸对兔卵巢颗粒细胞雌孕激素生成的影响  

Effect of Inhibitory and Excitatory Amino Acid on the Production of Estrogen and Progesterone by Granulosa Cells of Rabbits in Vitro

作　　者：张玮[1] 倪江[1] 程龙庆 张辉[1] 王京花[1] 张丽敏[1] 

机构地区：[1]哈尔滨医科大学生理教研室,哈尔滨150086

出　　处：《基础医学与临床》1999年第6期49-52,共4页Basic and Clinical Medicine

基　　金：黑龙江省自然科学基金!G93C-25-3

摘　　要：本实验采用离体孵育的兔卵巢颗粒细胞，观察四种氨基酸神经递质对颗粒细胞雌二醇和孕酮生成的影响。结果表明，γ-氨基丁酸和谷氨酸能明显刺激颗粒细胞在基础状况下雌二醉的分泌而抑制孕酮的分泌；甘氨酸和天门冬氨酸无此作用。γ-氨基丁酸、甘氨酸和谷氨酸明显促进hCG诱导的颗粒细胞雌二醇的分泌和抑制孕酮的分泌而；天门冬氨酸无此作用。发挥作用的机制可能与腺苷酸环化酶-cAMP系统有关。In the present experiment, the effects of GABA, Glu, Gly and Asp on the secretion of estradiol(E2)and progesterone (P)by cultured rabbit ovarian granulosa cells in vitro were investigated.The results showed that GABA and Glu could significantly stimulate estradiol and inhibite basal progesterone secretion. While Gly and Asp had no significant effect. In addition, GABA, Glu and Gly also promoted significantly secretion of E2 by granulosa cells as induced by hCG, while GABA and Gly inhibited secretion of P. Asp had no effect on production of E2 and P. GABA, Gly could stiumlated cAMP production, as suggesting that cAMP might serve as a 'second messenger'to regulate production of E2 and P.

关 键 词：谷氨酸 雌二醇 孕酮 颗粒细胞 氨基酸 卵巢 

分 类 号：R339.22[医药卫生—人体生理学]