人红细胞抗高血压因子对人脐静脉VSMC胞浆及核内Ca^(2+)水平的影响  

Effect of Antihypertensive Factor from Human Erythrocyte on Calcium Level in Human Umbilical Vein VSMC

作　　者：徐晓蓉[1] 张晓春[1] 文允镒[1] 

机构地区：[1]中国医学科学院基础医学研究所

出　　处：《基础医学与临床》1999年第6期58-62,共5页Basic and Clinical Medicine

基　　金：国家自然科学基金!39740026;中国医学科学院基金

摘　　要：采实验用Fluo-3／AM染色在激光扫描共聚焦显微镜下观察了红细胞抗高血压因子（antihypertensivefactor，AHF）对人脐静脉VSMC胞浆（［Ca2＋］i）及核内（［Ca2＋］n）游离钙离子水平的影响，结果表明：AHF（10-4g／mL）明显抑制Bayk8644（10-6mol／L），KCl（60mmol／L），AngⅡ（10-6mol／L），CPA（10-5mol／L）及IP3（10-5mol／L）引起的人脐静脉VSMC［Ca2＋］i与［Ca2＋］n升高。结果提示：1．AHF通过抑制Ca2＋内流、胞内钙库、放等机制阻断［Ca2＋］i的升高：2．AHF有益于核内钙稳态的维持。The effects ot antihypertensive factor(AHF) on the tranportaion of cytoplasmic [Ca2+]i and unclear ot leves([Ca2+]n)in human umbilical vein vascular smooth muscle cell (hvu VSMC ) were studied using laser scanning confocalmicroscopy together with Fluo-3/AM calcium indicator.The result indicated that AHF(10-4g/mL)inhibited the increase of intracellular free calcium concentraion ([Ca2+]i) and intranuclear free calcium concentration([Ca2+]n) induced by Bay-k8644 (10-6mol/L), KCl (60mmol/L),angiotensin Ⅱ(10-6mol/L), cyclopiazonic acid (CPA, 10-5mol/L) and inosito1-1 ,4,5-triphospate (IP3,10-5mol/L) significantly.The results sugges that:1, AHF might inhibit the abnomal rise in [Ca2+]ithrough multiple mechanisms including blocking of potential-dependent calcium channel (PDC) and resisting the release of Ca2+ from intracellular calcium pools. 2, AHF can diminish the increase of [Ca2+]n., this indicate tha it might inhibit the proliferation and apoptosis of VSMC in hypertension since a high [Ca2+]n. may be responsible for that.

关 键 词：AHF 胞浆游离钙 平滑肌细胞 AVSMC 

分 类 号：R972.4[医药卫生—药品]