氦氖激光联合黄芪注射液对小鼠骨髓造血细胞的影响  

THE EFFECTS OF HE-NE LASER COMBINING WITH UNION ASTRAGALUS INJECTION ON BONE MARROW HEMATOPOIETIC

作　　者：李仙峰[1] 崔明玉[1] 崔珈衔[2] 任明姬[1] 

机构地区：[1]内蒙古医学院组织胚胎学教研室,内蒙古呼和浩特010059 [2]内蒙古医学院基础医学院

出　　处：《内蒙古医学院学报》2011年第3期193-196,共4页Acta Academiae Medicinae Neimongol

基　　金：内蒙古自治区自然科学基金项目(200607010909)

摘　　要：目的:研究低强度氦氖激光及联合黄芪注射液对小鼠骨髓造血细胞的影响,为临床扩增造血干细胞提供新的方法和实验依据。方法:黄芪注射液与4.20 J/cm2氦氖激光刺激小鼠后,用流式细胞术检测各组的造血干细胞Sca-1阳性率的表达情况及骨髓造血细胞的周期变化。结果:各实验组小鼠骨髓造血干细胞Sca-1阳性率的表达及骨髓造血细胞周期中S期、G2/M期的细胞比例均明显增高,且激光照射与黄芪注射液联合组结果最显著。结论:低强度氦氖激光能够对小鼠骨髓造血细胞产生刺激增殖作用,且黄芪注射液与其有协同刺激作用。Objective： To investigate low intensity helium-neon laser combining with the union astragalus on mouse marrow hemopoiesis,to provide the new method for clinical increase the hematopoietic stem cell.Method： Astragalus injection after dosage 4.20 J/cm2 helium neon laser stimulation mouse,examines each group with the class type cell technique the hematopoietic stem cell Sca-1 masculine gender rate expression situation and the marrow hematopoietic cell cycle change.Result： Various experimental groups mouse marrow hematopoietic stem cell Sca-1 masculine gender rate expression and the marrow hematopoietic cell cycle of the S time,the G2/M time＇s cell proportion obviously advances,4.20 J/cm2 laser show and the astragalus injection union group result is most remarkable.Conclusion：The low intensity helium neon laser have the stimulation multiplication function to the mouse marrow hematopoietic cell,and the astragalus injection has the coordination stimulation with it.

关 键 词：氦氖激光 黄芪注射液 骨髓造血细胞 细胞周期 

分 类 号：R331.2[医药卫生—人体生理学] R318.5[医药卫生—基础医学]