不同地区不同生境按蚊种群的分子鉴定及rDNA-ITS2序列分析  

Molecule Identification and rDNA-ITS2 Sequence Analysis of Anopheles from Different Provinces and Habitats

作　　者：谭伟龙[1,2] 赵彤言[1] 董言德[1] 汪中明[1] 李春晓[1] 

机构地区：[1]军事医学科学院微生物流行病研究所,病原微生物生物安全国家重点实验室,北京100071 [2]南京军区疾病预防控制中心,南京210002

出　　处：《寄生虫与医学昆虫学报》2011年第2期84-89,共6页Acta Parasitologica et Medica Entomologica Sinica

摘　　要：对采自安徽、江苏、湖南、广西、云南及海南等地区的人房、牛棚、稻田、山区等不同生境的按蚊，按照文献合成引物，用PCR方法进行分子鉴定；并对各种群的ITS2序列测序后，与GenBank所得赫坎按蚊复合体近缘种群按蚊的ITS2序列进行比对分析。结果显示，采集的不同地区不同生境的按蚊均为中华按蚊，此次采集没有采到嗜人按蚊；现场的中华按蚊与凉山按蚊、贵阳按蚊和昆明按蚊的同源性为80．6％～83．1％．与嗜人按蚊和雷氏按蚊的同源性为75．2％～76．2％，与最黑按蚊和带足按蚊的同源性稍低，为63．2％～64．6％。系统发育树显示：所有的序列样本形成了两个分支，来自现场的按蚊处在同一分支且差异小。The partial rDNA-ITS2 segments of Anopheles from Jiangsu, Anhui, Hunan, Yunnan, Hainan provinces and Guangxi Zhuang autonomous region were amplified using PCR and then were sequenced. The sequence alignment results of rDNA-ITS2 indicated that all the samples obtained from different provinces and habitats belonged to Anopheles sinensis. There were high identities between the samples group which ranging from 98.2% to 100%. The identities between the samples and An. hycauns （An. liangshanensis, An. kunmingensis, An. lesteri and An. anrhropophagus, An. nigerrimus An. peditaeniatus） were 79. 1% -82% , 76. 1% -77. 1% , 60.9% -63.9% , respectively. Phylogenetic analysis results showed that all the segments obtained from the fields were clustered together and formed two separated branches with the segments of An. hycauns form GenBank.

关 键 词：按蚊 分子鉴定 RDNA-ITS2 序列分析 同源性 

分 类 号：Q969.442.2[生物学—昆虫学]