PCR扩增日本血吸虫Sj26、Sj32和Sj14-3-3抗原编码基因的诊断作用  

Diagnostic value of Sj26, Sj32 and Sj14-3-3 coding gene of Schistosoma japonicum amplified by PCR

作　　者：蔡世飞[1] 李文桂[1] 王敏[1] 

机构地区：[1]重庆医科大学附属第一医院传染病寄生虫病研究所,400016

出　　处：《中国地方病学杂志》2011年第4期361-363,共3页Chinese Jouranl of Endemiology

基　　金：重庆市科委地方病重大专项基金（2008AB5055、2008AB5008、2008AB5054）

摘　　要：目的 探讨PCR扩增日本血吸虫Sj26、Sj32和Sj14-3-3抗原编码基因的诊断价值.方法 从40例慢性日本血吸虫病患者血清中提取13本血吸虫DNA,用PCR扩增Si26、Sj32和sjl4-3-3抗原编码基因.1.2%琼脂糖凝胶电泳进行鉴定,同时以21例华支睾吸虫病、13例卫氏并殖吸虫病患者血清和43例健康人血清作对照.结果 从慢性日本血吸虫病患者血清中可扩增出长度为399 bp的Sil4-3_3抗原编码基因片段,但未扩增出长度为676 bp的Si26和1270 bp的Sj32抗原编码基因片段.对照组均呈阴性反应.结论 PCR扩增Sjl4-3-3抗原编码基因可用于慢性日本血吸虫病的基因诊断.Objective To investigate the diagnostic value of coding gene of Sj26, Sj32 and Sj14-3-3 amplified by PCR for chronic Schistosomiasis japonica. Methods The DNA was extracted from sera of 40 patients with chronic Schistosomiasis japonica, the coding gene of Sj26, Sj32 and Sj14-3-3 was amplified by PCR and identified by 1.2% agarose gel electrophoresis. DNA from the sera of 21 patients with Clonorchiasis sinensis, 13 patients with Parogonimiasis westermani and 43 healthy donors was taken as control. Results A total of 399 bp coding gene of Sj14-3-3 was amplified successfully from sera of the patients with chronic Schistosomiasis japonica,but Sj26（676 bp） and Sj32（ 1270 bp） coding gene were not obtained. Control groups were all negative. Conclusions Sj14-3-3 coding gene amplified by PCR can be used for genetic diagnosis of chronic schistosomiasis.

关 键 词：血吸虫病 日本 聚合酶链反应 基因疗法 

分 类 号：R686[医药卫生—骨科学]