HIV/HCV共感染者自然杀伤细胞表面活化性与抑制性受体表达的研究  

The studies for activating and inhibitory receptors on natural killer cells in HIV/HCV co-infected patients

作　　者：赵鹏[1] 张昕[1] 康富标[1] 张新伟 张柳 谭文辉 陈威巍 赵敏 

机构地区：[1]解放军军医进修学院,北京100853 [2]解放军第三零二医院感染性疾病诊疗与研究中心

出　　处：《中华实验和临床病毒学杂志》2011年第3期182-184,共3页Chinese Journal of Experimental and Clinical Virology

基　　金：首都医学发展科研基金（课题编号 2007-3036）;国家自然科学基金（课题编号81072423）

摘　　要：目的 比较HIV/HCV共感染、单纯HCV感染、单纯HIV感染者和健康人自然杀伤细胞（NK）数量及其表面受体的变化,了解HIV/HCV共感染者NK细胞表面活化性与抑制性受体表达特点.方法 采用流式细胞术对24例HIV/HCV共感染者,28例单纯HCV感染者,21例单纯HIV感染者外周血NK细胞数量与其表面活化与抑制性受体进行检测并与20例健康人进行比较分析.结果 HIV/HCV共感染组NK细胞绝对值较其他3组显著减少;共感染组、单纯HIV感染组、单纯HCV感染组NK细胞上NKP30和NKP46的表达频率都显著低于健康对照组,但NKP30的频率在前3组之间差异无统计学意义.共感染组和单纯HIV感染组的NKP46表达频率都显著低于HCV单纯感染组,而前2组之间差异无统计学意义;共感染组和单纯HCV感染组的NKG2A表达频率显著高于健康对照组和单纯HIV感染组,而前2组之间差异无统计学意义,但单纯HIV感染组NKG2A表达频率显著低于健康对照组;NK细胞NKG2D、CD158a和CD158b的表达频率在各组间差异无统计学意义.结论 HIV/HCV共感染者NK细胞绝对值明显降低,其表面活化性受体表达减少,某些抑制受体表达增加,甚至高于单纯HIV感染者,HIV/HCV共感染者NK细胞受损更加严重.Objective To investigate the characteristics of inhibitory and activating receptor expressions on natural killer （ NK） cells in HIV/HCV co-infected patients. Methods Numbers, frequencies and expressions of activating and inhibitory receptors of NK cells were measured with flow cytometry （FCS） from HIV/HCV co-infected group （n = 24）, HCV mono-infected group （n=34）, HIV mono-infected group （ n = 21 ） and healthy control group （ HC,n = 20） , then analysis and compare were performed among those groups. Results The NK cell absolute counts in HIV/HCV group were significantly lower than those in other three groups. The NKP30 and NKP46 frequencies on NK cells in HIV/HCV, HIV and HCV groups were all significantly lower than those in HC group, but there were no significant differences of NKP30 among former three groups; and NKP46 frequencies in HIV/HCV and HIV groups were lower than those in HCV group, but there were no significant differences between former two groups. The NKG2A frequencies in HIV/HCV and HCV groups were all higher than those in HIV and HC groups significantly, but the NKG2A frequencies in HIV group were lower than those in HC group; There were no significant differences of NKG2D,CD158a and CD158b among those four groups. Conclusion NK cell numbers and expressions of activiting receptors on NK cells obviously decreased in HIV/HCV co-infected patients, but some inhibitory receptors expressions increased, even higher than those of HIV mono-infected patients. NK cells impairments in HIV/HCV co-infection is more severe than HIV or HCV mono-infection.

关 键 词：HIV 肝炎病毒属 感染 杀伤细胞 天然 

分 类 号：R512.91[医药卫生—内科学]