中华稻蝗血细胞染色方法的比较  

Comparison among three staining methods to hemocytes of Oxya chinensis

作　　者：王强[1] 崔智芳[1] 王云丽[1] 申旭燕[1] 张小民[1] 

机构地区：[1]山西大学生命科学学院,太原030006

出　　处：《应用昆虫学报》2011年第4期841-844,共4页Chinese Journal of Applied Entomology

基　　金：国家自然科学基金(30770239);山西省自然基金(2009011048)

摘　　要：通过Giemsa、Wright和Giemsa-Wright混合染色3种方法,对中华稻蝗Oxya chinensis(Thunberg)的血细胞进行了染色和血细胞的形态学观察,其结果是Giemsa染液染色时间较长,对细胞核染色效果较好,细胞质界限清晰,但对细胞质中的颗粒染色较差;Wright染液染色时间较短,对细胞核和细胞质中的颗粒染色效果较好,但细胞质的界限不淸;Giemsa-Wright混合染色具备了2种染液的优点,时间介于两者之间,细胞核和细胞质中的颗粒染色效果较好,细胞界限清晰,该法可用于蝗虫血细胞,以及昆虫血细胞的广泛研究。The staining efficiency of the Giemsa,Wright and Wright-Giemsa staining methods were compared with respect to hemocytes of Oxya chinensis（Thunberg）.The Giemsa method effectively stained the nucleus;cytoplasm boundaries were clear,but it was relatively time consuming and did not stain cytoplasmic granules very well.The Wright method effectively stained both nucleus and cytoplasmic granules in a relatively short time,but cytoplasm boundaries were not clear.The Wright-Giemsa method had the advantages of both the Giemsa and Wright methods,including shorter staining time,good staining of nucleus and cytoplasmic granules and clear cytoplasm boundaries.This method should be used more widely in studying insect hemocytes.

关 键 词：中华稻蝗 血细胞 染色方法 

分 类 号：S433.2[农业科学—农业昆虫与害虫防治] Q962[农业科学—植物保护]