鉴定转Bt甘蔗抗条螟的方法  

Method to identify insect resistance of Bt-transgenic sugarcane lines

作　　者：劳方业[1] 翁丽星[2] 李奇伟[1] 陈勇生[1] 汪联辉[2] 陈健文[1] 陈仲华[1] 张琼[1] 沈万宽[1] 陈海宁[1] 邓海华[1] 

机构地区：[1]广州甘蔗糖业研究所广东省甘蔗改良与生物炼制重点实验室,广州510316 [2]复旦大学生命科学学院微生物学和微生物工程系,上海200433

出　　处：《应用昆虫学报》2011年第4期1098-1102,共5页Chinese Journal of Applied Entomology

基　　金：广东省教育部产学研结合项目(2008B090500227);广东省科技计划项目(2008B020200002);现代农业产业技术体系建设专项资金资助

摘　　要：通过采集蔗田条螟Procerasvenosatus Walker的蛹,在室内可控条件下进行羽化、交配、产卵、孵化的方式获得虫源,并将初孵幼虫接于检疫温室桶栽的13个转Bt基因甘蔗品系的心叶上,7d后调查幼虫的生长死亡情况以及甘蔗受害程度。结果表明,综合幼虫的生长死亡情况和甘蔗受害程度二项指标,能更准确评价甘蔗的抗虫性,13个转Bt基因甘蔗品系的抗虫性差异悬殊,从高抗到高感不等。该评价方法简单易行,结果准确可靠,可作为转Bt基因甘蔗抗虫性鉴定的一个主要方法。The relative resistance of 13 Bt-transgenic sugarcane lines to insect pests was investigated using plants planted in buckets in a quarantine greenhouse.Stem borer neonates Proceras venosatus Walker were obtained by collecting sugarcane borer pupae from cane fields and allowing these to undergo eclosion,mate,oviposit and hatch under controlled,indoor conditions.Newly hatched larvae were then inoculated into the leaf spindle of sugarcane plants.The growth and survival of larvae and the degree of plant damage was investigated 7 days after inoculation.The results show that two indicators,larval mortality and plant damage,provided the most accurate indication of the relative resistance of sugarcane varieties to insect browsing.The 13 transgenic sugarcane lines varied markedly in resistance to stem borers,some being highly resistant and others highly sensitive.This method is a simple and reliable way to compare the resistance of Bt-transgenic sugarcane lines to stem borer.

关 键 词：转Bt甘蔗 条螟 抗虫性 鉴定方法 

分 类 号：S435.661[农业科学—农业昆虫与害虫防治]