谷跗线螨交叉反应性变应原的定位  

Localization of cross-reactivity allergens in the body of Tarsonemus granarius (Acari: Tarsonemidae) by immunohistochemistry

作　　者：赵学影[1,2] 赵振富[2] 孙新[1] 刘志刚[2] 吕艳思[2] 

机构地区：[1]蚌埠医学院病原生物学教研室,感染与免疫安徽省重点实验室,安徽蚌埠233030 [2]深圳大学过敏反廊与免疫学研究所,广东深圳518060

出　　处：《昆虫学报》2011年第7期848-852,共5页Acta Entomologica Sinica

基　　金：国家“863”计划项目(2006AA100308);国家自然科学基金项目(81071388);深圳市科技计划三新重点项目(200902);深圳市科技计划项目(200945);深圳大学科技创新团队项目(200904)

摘　　要：为明确谷跗线螨Tarsonemus granarius交叉反应性变应原在体内的定位,制作谷跗线螨石蜡切片,HE染色后光镜观察其内部形态结构;选用对尘螨过敏患者的阳性血清特异性IgE抗体作探针,免疫组织化学染色分析其交叉反应性抗原存在位置。HE染色显示谷跗线螨的消化系统占据其体腔的大部分,包括口咽部、中肠、后肠、肛门和唾液腺等结构。免疫组织化学染色显示谷跗线螨的口咽部、中肠、肠内容物、肠壁及生殖腺等均有阳性反应。谷跗线螨体内存在可诱发人体IgE抗体的变应原,变应原在其体内的分布格局与屋尘螨Dermatophagoides pteronyssinus基本一致。To determine the localization of cross-reactivity allergens in the body of Tarsonemus granarius, paraffin sections of T. granarius stained by hatmatoxylin-eosin （HE） were observed by optical microscope. Serum specific IgE antibodies from patients who were sensitive to the house dust mite served as probe, and the cross-reactivity allergens in the body of T. granarius were localized by the method of immunohistochemistry. The digestive system was found to occupy large parts of body cavity of T. granarius by HE staining, which consists of oropharynx, midgut, hindgut, anus, salivary gland and so on. The cross-reactivity allergens of T. granarius mostly occurred in the midgut tissue, gut contents, cuticle and reproductive system in the immunostained sections. The body of T. granarius has allergens which can trigger IgE antibodies of the human body and the distribution of allergens in its body is consistent with that in the house dust mite, Dermatophagoides pteronyssinus.

关 键 词：谷跗线螨 跗线螨科 变应原 抗原定位 免疫组织化学 HE染色 交叉反应性 

分 类 号：Q965[生物学—昆虫学]