棉花叶绿体基因RNA编辑位点的测定及分析  

Identification and Analysis of RNA Editing Sites in Chloroplast Transcripts of Gossypium hirsutum

作　　者：江媛[1] 范术丽[2] 宋美珍[2] 俞嘉宁[1] 喻树迅[2] 

机构地区：[1]陕西师范大学生命科学学院,西安710062 [2]中国农业科学院棉花研究所/农业部棉花遗传改良重点实验室,安阳455000

出　　处：《植物学报》2011年第4期386-395,共10页Chinese Bulletin of Botany

基　　金：国家重点基础研究发展计划(No.2010CB126006);转基因生物新品种培育(No.2008ZX08005-002)

摘　　要：陆生植物叶绿体RNA编辑是转录后基因表达调控的一种重要方式。该文在预测棉花(Gossypium hirsutum)叶绿体基因RNA编辑位点的基础上,选取中棉10(CRRI 10)为实验材料,采用PCR、RT-PCR及测序等方法,确定CRRI 10的27个叶绿体蛋白编码基因共有55个编辑位点,均是C→U的转换。与棉种柯字310(C310)的编辑位点比对后发现,CRRI 10多出accD-468和rpoC1-163两个编辑位点,同时缺失psbN-10。利用生物信息学分析这3个位点,rpoC1-163和psbN-10的编辑可能会改变各自蛋白的二级结构。对CRRI 10中55个编辑位点上游的顺式作用元件(?30–?1)分析显示,共有8组顺式作用元件的相似性达到60%或以上,推测各组中的编辑位点可能由相同的反式作用因子来识别。RNA editing is one of the post-transcriptional modification processes in which the bases of a RNA molecule are altered by the addition,deletion and alteration of nucleotides.In most higher plants,RNA editing mainly occurs in mitochondria and plastids and converts from C to U,very rarely from U to C.We investigated RNA editing sites in chloroplasts of Gossypium hirsutum ‘CRRI 10’ by PCR,RT-PCR and sequence alignment.We identified 55 editing sites in 27 protein-coding genes all of which were C-to-U conversion.By comparing editing sites between CRRI 10 and Coker310FR,CRRI 10 had two novel editing sites,accD-468 and rpoC1-163,whereas site psbN-10 was absent.Bioin-formatics analysis of the 3 sites revealed that rpoC1-163 and psbN-10 editing might affect the secondary structure of the corresponding protein.Comparison among upstream regions（？30 to ？1） of the 55 editing sites of CRRI 10 revealed that 8 pairs share more than 60% sequence similarity suggesting that the sites in each pair may be recognized by the same trans-acting factors.

关 键 词：叶绿体 顺式作用元件 棉花 RNA编辑 

分 类 号：S562[农业科学—作物学] Q943[生物学—植物学]