rSj26-Sj32融合蛋白用于慢性日本血吸虫病的诊断研究  

Study on diagnosis of chronic schistosomiasis japonicum with rSj26-Sj32 fusion protein

作　　者：蔡世飞[1] 李文桂[1] 王敏[1] 

机构地区：[1]重庆医科大学附属第一医院传染病寄生虫病研究所,重庆400016

出　　处：《细胞与分子免疫学杂志》2011年第8期839-842,共4页Chinese Journal of Cellular and Molecular Immunology

基　　金：重庆市科委地方病重大专项基金资助(2008AB5055;2008AB5008;2008AB5054)

摘　　要：目的:探讨rSj26-Sj32融合蛋白用于慢性日本血吸虫病的诊断价值。方法:用rSj26-Sj32融合蛋白和日本血吸虫成虫粗抗原(SjAWA)作为包被抗原建立酶联免疫吸附试验(ELISA)、斑点酶联免疫吸附试验(Dot-ELISA)和dipstick三种方法检测慢性日本血吸虫病患者血清特异性IgG抗体,同时以华支睾吸虫病、卫氏并殖吸虫病、泡型棘球蚴病和囊型棘球蚴病患者以及健康人血清作为对照。结果:rSj26-Sj32融合蛋白通过三种方法检测慢性日本血吸虫病的敏感性分别为95.00%、92.50%和92.50%;特异性分别为97.67%、95.35%和97.67%。结论:rSj26-Sj32融合蛋白可用于慢性日本血吸虫病的免疫诊断。AIM： To study whether rSj26-Sj32 fusion protein can be used for diagnosis of chronic schistosomiasis japonicum.METHODS： ELISA,Dot-ELISA and dipstick were performed to detect the IgG in the sera of patients with chronic schistosomiasis japonicum using the antigens of rSj26-Sj32 fusion protein and SjAWA.The control sera were taken from health donors and the patients with clonorchiasis sinensis,paragonimiasis westermani,alveolar echinococcosis and cystic echinococcosis.RESULTS： The sensitivity of the three methods was 95.00%,92.50% and 92.50% respectively,whereas the specificity of the three methods was 97.67%,95.35% and 97.67%,respectively.CONCLUSION： The rSj26-Sj32 fusion protein has therapeutic potential for immunodiagnosis of chronic schistosomiasis japonicum.

关 键 词：日本血吸虫病 免疫诊断 融合蛋白 斑点酶联免疫吸附试验 dipstick法 

分 类 号：R392.33[医药卫生—免疫学]