血清IFN-γ及RANTES水平与兔动脉粥样硬化易损斑块的相关性  

Study on the correlation of the levels of serum IFN-γ and RANTES and rabbit vulnerable atherosclerotic plaque

作　　者：问海燕[1,2] 彭昱东[2] 程龙献[2] 

机构地区：[1]武汉理工大学校医院内科,湖北武汉430063 [2]华中科技大学同济医学院附属协和医院心血管病研究所,湖北武汉430022

出　　处：《细胞与分子免疫学杂志》2011年第8期846-848,共3页Chinese Journal of Cellular and Molecular Immunology

摘　　要：目的:研究血清IFN-γ及调节激活正常T细胞表达和分泌趋化因子(RANTES)水平与家兔动脉粥样硬化易损斑块之间的相关性。方法:30只雄性家兔随机分为3组:①空白对照组(control组,10只),普通饲料喂养12周;②稳定斑块组(AS组,10只),高脂饮食(含胆固醇1%和猪油5%)喂养12周;③易损斑块组(VAP组,10只)高脂饮食喂养12周,于处死前24 h和48 h分别给予两次药物触发。测定各组第0周、12周血脂,12周处死时检测兔血清INF-γ和RAN-TES水平,Masson染色法计算校正的斑块面积、易损指数及其与血清炎症因子的相关系数。结果:AS组和VAP组血清IFN-γ水平(2.95±0.92;3.77±0.51)均显著高于control组(1.52±0.23,P<0.01),VAP组血清IFN-γ明显高于AS组(P<0.01)。AS组血清RANTES水平较Control组无统计学差异(26.01±4.45vs25.88±4.22;P>0.05),VAP组血清RANTES水平(96.48±11.22)明显高于AS组和Control组(P<0.01)。VAP组校正的斑块面积显著高于AS组(51.26±12.58vs31.26±8.76,P<0.01),VAP组易损指数显著高于AS组(7.25±1.87vs3.14±1.22,P<0.01)。AS组和VAP组血清IFN-γ、RANTES水平分别与校正的斑块面积和易损指数呈正相关(P<0.05)。结论:可能是两个监测动脉粥样硬化斑块易损性的炎症标记物。IFN-γ和RANTES血清表达水平与斑块的稳定性有一定关系。AIM： To investigate the correlation between the levels of serum IFN-γ and RANTES（regulated upon on activation on normal T cell expressed and secreted） and rabbit atherosclerotic plaque stability.METHODS： 30 male New Zealand rabbits were randomly divided into three groups： control group,stable atherosclerotic plaque group（AS group） and vulnerable atherosclerotic plaque group（VAP group）,with 10 rabbits in a group.The control group was given normal diet while the AS group and the VAP group were given high-fat diet for 12 weeks.According to literature method pharmacological triggers inducing the vulnerable atherosclerotic plaque formation in the VAP group.Fasting blood was extracted from ear medium artery about 2 mL at week-0 and the 12th week,serum total cholesterol,triglyceride and high density lipoprotein cholesterol were detected by enzymatic method,and low density lipoprotein cholesterol was calculated by Friedwald formula.All animals were executed after they were fed by injected excessive Phenobarbital sodium at ear vein for 12 weeks.Before executed,ear medium artery blood was taken to detect the levels of serum IFN-γ and RANTES by ELISA.The corrected plaque area and vulnerability index were calculated by Masson staining and its correlation coefficient with inflammatory factors were measured.RESULTS： The level of serum IFN-γ at the AS group and the VAP group,compared with the control group,was significantly increased（all P〈0.01）,at the same time,the level of serum IFN-γ in the VAP group was significantly higher than in the AS group（P〈0.01）.About the level of serum RANTES there was no obvious difference when the AS group was compared with the control group（P〉0.05）.The level of serum RANTES in the VAP group was higher than in the AS group and the control group（all P〈0.01）.Regarding corrected plaque area and vulnerability Index,the VAP group was significantly higher than the AS group（P〈0.01）.In the AS group and the VAP group,the serum concentrations of IFN-γan

关 键 词：Γ-干扰素 RANTES 动脉粥样硬化 易损斑块 

分 类 号：R392.11[医药卫生—免疫学]