脂肪来源的干细胞移植对脑缺血大鼠神经轴突再生的影响  

Outgrowth of neuronal axons on adipose-derived stem cell transplanting for treatment of cerebral infarction in rats

作　　者：陈爱珍[1] 刘楠[1] 黄欢[1] 林菲菲[1] 刘德山[1] 林小惠[1] 

机构地区：[1]福建医科大学附属协和医院神经内科,福建福州350001

出　　处：《细胞与分子免疫学杂志》2011年第8期868-871,共4页Chinese Journal of Cellular and Molecular Immunology

基　　金：福建省自然科学基金资助项目(2008J02822008J0283)

摘　　要：目的:探讨脂肪来源的干细胞(ADSC)移植对脑缺血大鼠神经轴突生长以及神经胶质酸性蛋白(GFAP)、神经突蛋白(Neuritin)、神经微丝蛋白200(NF-200)表达的影响。方法:54只清洁级成年雄性SD大鼠,随机分为3组:假手术组(Sham组)、模型组(MCAO组)及MCAO+ADSC治疗组(ADSC组),每组18只。采用改良Zea-Longa线栓制法大脑中动脉栓塞(MCAO)模型,ADSC移植前用DAPI标记,ADSC组于造模成功1 d后经侧脑室注射入ADSC(1×106),分别于术后7 d、14 d、28 d观察其恢复情况,并断头取脑,通过免疫荧光、Western blot法检测脑缺血组织中GFAP、Neuritin、NF-200表达情况。结果:ADSC组缺血周边区脑组织中能观察到DAPI染色的阳性细胞;ADSC组与MCAO组相比在各个时间点脑组织中GFAP阳性细胞表达明显降低(P<0.05),神经突蛋白和神经微丝蛋白200表达明显增高(P<0.05)。结论:ADSC移植后可引起脑缺血后期组织中Neuritin、NF-200有效表达,并抑制GFAP阳性细胞增生,促进了神经轴突再生和修复。AIM： To investigate the effects of adipose-derived stem cell（ADSC） transplanting on the outgrowth of neuronal axons and the expressions of GFAP,Neuritin,NF-200 in the brain post focal cerebral ischemia in rats.METHODS： 54 male adult Sprague-Dawley rats were randomly divided into 3 groups： sham-operated group,middle cerebral artery occlusion（MCAO） group and MCAO＋ADSC-treated group（n=18 in each group）.A permenant focal cerebal ischemia model was established using modified Longa＇s method ADSC was labeled by DAPI before the transplantation.One day after MCAO,30 μL of cell suspension containing 1×10^6 cells were injected into the lateral ventricle of MCAO＋ADSC-treated group.At 7 d,14 d and 28 d after MCAO,the expressions of GFAP,Neuritin and NF-200 were detected in ischemic region by Western blot and Immunofluorescence analysis.RESULTS： DAPI staining positive cells were observed around the cerebral infarcted area in the ADSC group.The expressions of Neuritin,NF200 were higher,but GFAP was lower than that of the MCAO group at 7 d,14 d and 28 d（P〈0.05）.CONCLUSION： The transplantation of ADSC can induce regeneration and repairment of impaired neuronal axons in rat brain after cerebral ischemia,partly by inhibiting the expression of GFAP and enhancing the expressions of Neuritin,NF-200 in the brain.

关 键 词：ADSC MCAO 神经轴突 神经胶质酸性蛋白 神经突蛋白 神经微丝蛋白200 

分 类 号：R392.12[医药卫生—免疫学]