Forskolin对昆明白小鼠卵母细胞体外成熟及卵丘扩展的影响  

The Effect of Forskolin on Kunming White Mouse Oocyte Maturation and Cumulus Expansion \%In vitro\%

作　　者：陈勇[1] 夏国良[1] 苏友强[1] 王海滨[1] 李美玲[1] 

机构地区：[1]中国农业大学生物学院,北京100094

出　　处：《农业生物技术学报》1999年第3期219-222,共4页Journal of Agricultural Biotechnology

基　　金：国家教委回国人员启动基金;国家自然科学基金

摘　　要：初步探讨了在体外培养条件下ｆｏｒｓｋｏｌｉｎ 对昆明白小鼠卵母细胞成熟和卵丘扩展的影响。培养体系中存在ＨＸ 时，ｆｏｒｓｋｏｌｉｎ 能显著促进卵丘卵母细胞复合体（ＣＥＯ） 的卵丘扩展，但对其卵母细胞成熟（ 即ＧＶＢＤ） 无明显影响。没有ＨＸ 存在时，ｆｏｒｓｋｏｌｉｎ 在培养期的１ ～６ ｈ 内可抑制ＧＶＢＤ 发生，该作用呈剂量依赖性，１０μｍｏｌｆｏｒｓｋｏｌｉｎ 完全抑制ＧＶＢＤ，但培养至２４ ｈ ，ｆｏｒｓｋｏｌｉｎ 对卵母细胞ＧＶＢＤ 的抑制作用完全消失。实验结果表明：体外培养条件下，ｆｏｓｋｏｌｉｎ 具有明显的促进卵丘扩展的作用， 但在培养早期却能抑制卵母细胞成熟。The effect of forskolin on Kunming white mouse oocyte maturation and cumulus expansion \%in vitro\% was studied. Cumulus expansion was significantly promoted by forskolin in cumulus cell enclosed oocyte complexes(CEO)cultured in medium containing hypoxanthine(HX),but no change in oocyte maturation was observed. In the absence of HX,forskolin inhibited the spontaneous resumption of meiosis in a dose dependent manner during the first 6 h of culturing,especially In 10 μmol forskolin dose group. After 24 h of culture,the inhibitory effect of forskolin disappeared. These results indicate that forskolin can stimulate cumulus expansion \%in vitro\%,but inhibit oocyte maturation of CEO in the early period of culture.

关 键 词：昆明 白小鼠 卵丘扩展 卵母细胞成熟 

分 类 号：Q959.837[生物学—动物学] Q492.5