绞股蓝总皂苷对NCTC11637株HP延缓大鼠实验性胃溃疡愈合的治疗作用及其机制  

Therapeutic role and its mechanism of gypenosides on delayed healing of experimental gastric ulcer induced by NCTC11637 strain HP in rats

作　　者：张青蓓[1,2,3] 马俊江[1,2,3] 曹之宪 林志彬[1,2,3] 

机构地区：[1]北京医科大学药理学系 [2]重庆医科大学药理学教研室 [3]香港大学药理学系

出　　处：《中国药理学通报》1999年第3期225-228,共4页Chinese Pharmacological Bulletin

基　　金：高等学校博士学科点专项科研基金

摘　　要：目的探讨绞股蓝总皂苷（ｇｙｐｅｎｏｓｉｄｅｓ，ＧＰ）对ＣａｇＡ（＋），ＶａｃＡ（＋）ＮＣＴＣ１１６３７株幽门螺杆菌（Ｈｅｌｉｃｏｂａｃｔｅｒｐｙｌｏｒｉ，ＨＰ）延缓动物实验性胃溃疡愈合的治疗作用及其机制。方法用醋酸诱发大鼠实验性胃溃疡，以溃疡面积、溃疡面积占腺胃部百分比、粘膜组织内白细胞介素８（ＩＬ８）、ＰＧＥ２、ＭＤＡ、ＳＯＤ、·ＯＨ及溃疡愈合时间为指标，观察ｉｇ给予冻干ＮＣＴＣ１１６３７ＨＰ的影响及给予ＨＰ１ｈ后ｉｇＧＰ的治疗作用。结果单给ＨＰ组，溃疡面积加大，愈合延迟，粘膜组织内ＩＬ８、ＭＤＡ升高，ＳＯＤ活性下降；·ＯＨ生成无明显变化；损伤粘膜组织内ＩＬ８升高，ＰＧＥ２亦同时升高。ＨＰ＋ＧＰ组溃疡面积、溃疡面积百分率明显减小；粘膜内ＭＤＡ、·ＯＨ生成抑制，ＩＬ８、ＰＧＥ２平行下降，ＳＯＤ活性提高。结论ＮＣＴＣ１１６３７株ＨＰ可明显延缓醋酸性大鼠胃溃疡的愈合；绞股蓝总皂苷通过抑制炎症反应过程中ＩＬ８、ＭＤＡ、·ＯＨ生成，并通过提高ＰＧＥ２和ＳＯＤ活性增强胃粘膜保护机制，对感染ＮＣＴＣ１１６３７株ＨＰ大鼠实验性胃溃疡产生显著治疗作用。AIM To investigate the adverse action of Helicobacter pylori (HP) (NCTC11637 strain) and the therapeutic effect of Gypenosides (GP) on ulcer healing in rats. METHODS After ulcer induction by acetic acid in the stomach, rats were divided into 4 groups. They were either given ig with distilled water or with CagA (+) and VacA(+) NCTC11637 HP (1 mg·kg -1 ·d -1 ) alone or together with GP (15 or 45 mg·kg -1 ·d -1 ) for 9 days. The changes of ulcer size, IL 8, PGE 2, MDA, SOD and ·OH in the gastric mucosa were assessed. RESULTS Administration of HP significantly increased the ulcer sizes and mucosal levels of IL 8, PGE 2 and MDA. The SOD activity reduced. These changes were alleviated by GP treatment. CONCLUSION NCTC11637 strain of HP could delay ulcer healing. GP administration reversed this adverse action by inhibiting the inflammatory responses, including IL 8, lipid peroxidation and hydroxyl free radical formation in the gastric mucosa. It also increased the protective effects through the stimulating action on SOD activity in the stomach.

关 键 词：胃溃疡 绞股蓝 幽门螺杆菌 皂甙 治疗 

分 类 号：R282.71[医药卫生—中药学] R573.1[医药卫生—中医学]