低氧激活海马神经元上ATP敏感性钾通道  

Hypoxia activates an ATP sensitive potassiunm channel in hippocampal neurons

作　　者：单红蕖[1] 郑平[1] 程介士[1] 

机构地区：[1]上海医科大学医学神经生物学国家重点实验室

出　　处：《中国药理学通报》1999年第3期257-259,共3页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金

摘　　要：目的观察ＡＴＰ敏感性钾通道（ＫＡＴＰ通道）在低氧时的状态及细胞内高浓度ＡＴＰ对它的影响。方法采用膜片钳全细胞及单通道两种方法直接证实低氧时ＫＡＴＰ通道的开放。结果低氧时ＫＡＴＰ通道开放，且这种开放状态可被细胞内注射５ｍｍｏｌ·Ｌ－１ＡＴＰ所阻断。ＫＡＴＰ通道开放剂与低氧时激活的通道类似。单通道记录发现，低氧后通道开放的频率、幅度明显加大。结论低氧时外向钾电流明显增加，它来源于ＫＡＴＰ通道的开放。AIM The state of K ATP channel and effect of intracellular high concentration of ATP on it during hypoxia were studied. METHOD Using whole cell and single channel recordings, we directly proved that K ATP channels were opened during hypoxia. RESULTS K ATP channels were opened during hypoxia and it was abolished by intracellular injection of 5 mmol·L -1 ATP. The frequence and amplitude of K ATP channel were increased obviously. CONCLUSION Outward K + currents elevated significantly and come from K ATP channel during hypoxia.

关 键 词：低氧 KATP通道 膜片钳 海马 神经元 脑缺血 

分 类 号：R322.81[医药卫生—人体解剖和组织胚胎学] R743.31[医药卫生—基础医学]