2005年-2009年浙江省甲1亚型流感病毒株NA区基因特性分析  

Genetic analysis of NA gene of influenza virus subtype A1 isolates in Zhejiang province from 2005 to 2009

作　　者：李敏红[1] 徐昌平[1] 卢亦愚[1] 茅海燕[1] 周敏[1] 

机构地区：[1]浙江省疾病预防控制中心,杭州310051

出　　处：《中国卫生检验杂志》2011年第7期1712-1714,共3页Chinese Journal of Health Laboratory Technology

摘　　要：目的:分析2005年-2009年浙江省甲1亚型流感流行株神经氨酸酶(NA)基因的特性、变异与进化特征。方法:选择浙江省2005年-2009年流感流行期间分离的甲1亚型流行代表株,提取病毒RNA,扩增病毒NA基因,进行序列测定,并用BioEdit、MEGA软件分析处理。结果:浙江省近几年甲1亚型流感流行株,所测NA区的核苷酸长度为1278 bp,编码426个氨基酸。甲1亚型流感病毒NA区的氨基酸同源性在96.0%～99.1%。这5年间NA区发生了4～10个氨基酸的替代变异,但是在氨基酸进化树上没有形成独立的分支。近几年的甲1亚型毒株NA区有7个糖基化位点,糖基化位点没有大的改变。结论:近几年来浙江省甲1亚型流感NA基因区域发生了一定程度的变异,流感病毒NA区的变异对于流感流行的影响值得密切关注。Objective：To analyze the genetic characteristics,variation and evolution of NA gene of influenza virus subtype A1 isolates in Zhejiang province from 2005 to 2009.Methods：The NA gene of influenza virus subtype A1 isolates in Zhejiang province from 2005 to 2009 were sequenced,the molecular and evolution characterization of these virus strains were analyzed by BioEdit and Mega 3.0 bioinformatics software.Results：NA regions of all the isolates were all 1278 bp,encoding protein of 426 amino acids.Isolates shared amino acid homology is 96.0%～99.1% in NA regions.During the 5 years 4 to 10 amino acids on NA region were substituted respectively,but phylogenetic analysis showed that there is no absolutely different branches.Additionally,all the isolates have 7 N-linked glyeosylation sites in NA region,with no significant divergences.Conclusion： There is a certain antigenic variation on NA gene of influenza virus subtype A1 in Zhejiang province from 2005 to 2009.More attention should be paid to divergence of NA regions of influenza virus owning to the significant effect to influenza epidemic.

关 键 词：流感病毒 甲1亚型 神经氨酸酶 基因特性 

分 类 号：R373.13[医药卫生—病原生物学]