三种检测方法对活动性结核病的临床诊断价值  

Clinical value of three methods in diagnosis of tuberculosis

作　　者：戴佩佩[1,2] 裘晓乐[3] 

机构地区：[1]温州医学院定理临床学院 [2]温州市第二人民医院检验科,浙江温州325000 [3]温州医学院附属第二医院输血科,浙江温州325000

出　　处：《中国卫生检验杂志》2011年第7期1748-1749,1752,共3页Chinese Journal of Health Laboratory Technology

摘　　要：目的:比较荧光定量PCR、抗酸染色和结核抗体检测结核分枝杆菌的灵敏度和特异性,及在临床诊断中的应用价值。方法:对91例肺结核病患者和102例非结核呼吸系统疾病患者的痰标本应用荧光定量PCR法和涂片抗酸染色法检测,支气管灌洗液标本抗酸染色法检测,并分离患者外周血血清检测结核分枝杆菌抗体,对3种检测方法的结果进行分析比较。结果:荧光定量PCR法、支气管灌洗液涂片抗酸染色、痰涂片抗酸染色、结核抗体检测阳性率分别为96.7%、47.3%、33.0%、93.4%,特异性分别为98.0%、97.1%、97.1%、92.2%。结论:荧光定量PCR和结核抗体检测对于涂片抗酸染色阴性的活动性肺结核患者有重要的诊断价值。Objective：To compare the sensitivity and specificity of the three methods（acid-fast stain,serum tuberculosis anti-body and fluorescence quantitative PCR）in the detection of mycobacterich tuberculosis.And also to evaluate them in the clinical diagnsis.Methods： The sputum specimens from 91 TB patients and 102 non-tuberculosis patients with respiratory system disease were detected by fluorescence quantitative PCR,the smear acid-fast dyes and acid-fast staining method of bronchial lavage fluid specimens,and the peripheral blood serum from these patients were isolated to detect the M.tuberculosis antibody.The results of the there methods were compared.Results： The positive rate of fluorescence quantitative PCR,the acid-fast staining method of bronchial lavage fluid specimens and sputum smear,tuberculosis antibody were 96.7%,47.3%,33.0% and 93.4% respectively,while the specificity of the four methods were 98.0%,97.1%,97.1% and 92.2% respectively.Conclusion： The detection of fluorescence quantitative PCR and tuberculosis antibody is helpful for the diagnosis of the active TB patients,whose smear acid-fast stain were negative.

关 键 词：结核分枝杆菌 结核病 抗酸染色 结核抗体 荧光定量PCR 

分 类 号：R52[医药卫生—内科学]