致医院感染金黄色葡萄球菌β-内酰胺酶产生率与噬菌体关系的探讨  

Exploration of relationship between production rate of beta-lactamase and bacteriophage in staphylococcus aureus of nosocomical infection

作　　者：马艳华[1] 刘连红[1] 罗建祥[1] 

机构地区：[1]武警广东省总队医院检验科,广州510507

出　　处：《中国卫生检验杂志》2011年第7期1755-1756,共2页Chinese Journal of Health Laboratory Technology

基　　金：广州市科技重点攻关项目(98-2-01-022)

摘　　要：目的:探讨金黄色葡萄球菌(以下简称金葡菌)β-内酰胺酶产生率和噬菌体之间的关系,掌握金葡菌在医院感染状况,为控制院内感染提供有力依据。方法:经API STAPH条进行金葡菌鉴定,同时用玻片法及试管法凝固酶试验进一步确认,用头孢硝噻吩纸片测定β-内酰胺酶,再用噬菌体进行分型。结果:金葡菌产β-内酰胺酶株占545株,噬菌体分布在Ⅰ群52A、79、80型,Ⅱ群55、71型;Ⅲ群47、77、83A型和不定型6株。金葡菌不产β-内酰胺酶占54株。噬菌体分布在Ⅰ群29型、Ⅱ群3B型和Ⅲ群4E型。结论:医院感染的金葡菌主要以产β-内酰胺酶株为主,并与噬菌体型别存在着密切的关系。Objective：To explore the relationship between production rate of beta-lactamase and bacteriophage in staphylococcus aureus,grasp the states of S.aureus infection in hospital,and provide helpful basis to control nosocomical infection.Methods： The S.aureus were screened by API STAPH reagent kit and identified by coagulase test（slide test and tube test）and beta-lactamase was detected by nitrocefin and then typed by its bacteriophage.Results： Beta-lactamase positive strains of S.aureus were 545,and their bacteriophage were distributed with type52A,type 79 and type 80 in group I,type 55 and type 71 in group II,type 47,type 77,type 83A in group III and 6 indefinite strains.Beta-lactamase negative strains of S.aureus were 54,and their bacteriophage were distributed with type 29 in group Ⅰ,type 3B in group Ⅱ,type 4E in group Ⅲ.Conclusion： Most of the hospital infection S.aureeus were beta-lactamase positive and closely related to their bacterio-types.

关 键 词：金葡菌 Β-内酰胺酶 噬菌体 医院感染 

分 类 号：R378.11[医药卫生—病原生物学]