一株高效秸秆纤维素降解真菌的分离、鉴定及系统发育分析  

Isolation,Identification and Phylogenetic Analysis of an Efficient Straw Cellulose-Degrading Fungus

作　　者：李方正[1] 唐亮[1] 赵建文[1] 王文娟[1] 赵宏坤[1,2] 

机构地区：[1]山东农业大学动物科技学院,山东泰安271018 [2]青岛农业大学食品科学与工程学院/山东省肉品质量安全控制工程技术研究中心,山东青岛266109

出　　处：《山东农业科学》2011年第7期5-8,共4页Shandong Agricultural Sciences

基　　金：山东省肉品质量安全控制工程技术研究中心组建项目资助

摘　　要：通过刚果红染色法初筛,结合产酶活性测定复筛,从奶牛、山羊瘤胃内容物中分离到一株纤维素高效降解菌NL-3,克隆了该菌的18S rDNA序列,并以其同源性为基础构建了相关属种的系统发育树。结果表明,18S rDNA序列全长504 bp,Blast显示该菌株与青霉菌属同源;NL-3菌株在进化关系上也与青霉菌属(Penicillium)聚成一族。特别是与Penicillium decumbensstrain JU-A10的同源性最高,序列相似性达99%。结合菌株形态学及18S rDNA基因序列分析结果对菌株进行鉴定,初步鉴定为青霉属的斜卧青霉(Penicillium decumbens)。A fungal strain named as NL- 3 that could degrade cellulose was isolated from the rumen of cows and sheep. The 18S rDNA gene sequence for strain NL- 3 was amplified and sequenced. The results showed that the complete 18S rDNA gene sequence was 504 bp. A phylogenetic tree was constructed by comparing the 18S rDNA sequence with other relative fungus species in the GenBank database. The strain NL -3 and Penicillium decumbens strain JU -A10 constituted a branch with the similarity value as 99%. According to the morphological characteristics and phylogenetic analysis, the strain NL- 3 was identified as Penicillium decumbens.

关 键 词：纤维素降解真菌 鉴定 系统发育分析 

分 类 号：Q93-331[生物学—微生物学]