腹水TNF-α,IL-6,IL-8测定及其临床价值  

PPA RESEARCH ON THE CLINICAL VALUE OF TNF-2, IL-6AND IL-8 ASSAY IN ASCITES

作　　者：丁一娟[1] 于皆平[1] 罗和生[1] 

机构地区：[1]湖北医科大学附属第一医院消化内科,430060

出　　处：《中国现代医学杂志》1999年第12期1-2,共2页China Journal of Modern Medicine

摘　　要：该研究应用单抗夹心酶联免疫吸附试验（ＥＬＩＳＡ）对１１８例腹水患者的腹水ＴＮＦ－α、ＩＬ－６、ＩＬ－８水平进行测定，探讨其对肝硬化腹水并自发性腹膜炎的诊断和鉴别诊断价值。结果肝硬化腹水并自发性腹膜炎（ＳＢＰ）组其腹水三种细胞因子均明显高于其它三组，特别是与低蛋白血症腹水组比较有高度显著差异（Ｐ＜０．０１），与肝硬化腹水组及腹膜转移癌腹水组比较有显著性差异（Ｐ＜０．０５），此两组与低蛋白血症腹水组比较有显著性差异（Ｐ＜０．０５）。而肝硬化腹水组与腹膜转移癌腹水组两者无差异（Ｐ＞０．０５）。表明ＴＮＦ－α、ＩＬ－６、ＩＬ－８水平检测对肝硬化腹水并自发性腹膜炎的诊断和鉴别诊断具有重要参考价值，但均不能作为鉴别良恶性腹水的指标。Objective: To explore the diagnostic and differential. diagnostic value of TNF-2, IL-6 and IL-8 in ascites due to cirrhosiscomplicated with spontaneous peritonitis. Methods: In 118 cases, the levels of TNF-2, IL-6 and IL-8 in ascites were measured byMonoantibody-sandwich ELISA. Results: The levels of TNF-2, IL-6 and IL-8 in the group of cirrhosis ascites complicated withspontaneous peritonitis were significantly higher than those in the group of hypoproteinemia (P<0.01), and than those in both thegroup of cirrhosis ascites and the group of ascites due to peritoneal metastatic carcinoma (P<0.05). The levels of these threecytofactors the last two groups were significantly higher than those in the group of hypoproteinemia (P<0.05). There was nosignificant difference of TNF-2, IL-6 and IL-8 between the cirrhosis ascites group and the metastatic carcinoma group (P>0.05).Conclusions: The results suggest that the assay of the levels of TNF-2, IL-6 and IL-8 is of great reference value for the diagnosisand deferential diagnosis of cirrhosis ascites complicated with spontaneous peritonitis, but not a differentiation index of identifyingbenign ascites from malignant ascites.

关 键 词：诊断 腹水 

分 类 号：R442.5[医药卫生—诊断学]