聚焦超声辐照百会穴对HIBD大鼠的神经康复作用  

Effect on Neurorehabilitation of HIBD Rats after Irradiation with Focused Ultrasound to the Bai Hui Acupoint

作　　者：廖丛[1] 周江堡[1] 胡文静[1] 李东[2] 司维[2] 王智彪[2] 张晓萍[3] 

机构地区：[1]重庆医科大学附属儿童医院神经内科,重庆市400014 [2]重庆医科大学生物医学工程系超声医学工程省部共建国家重点实验室 [3]重庆医科大学附属儿童医院儿科研究所,重庆市400014

出　　处：《中国超声医学杂志》2011年第7期591-594,共4页Chinese Journal of Ultrasound in Medicine

摘　　要：目的探讨聚焦超声穴位治疗对HIBD大鼠神经康复的影响。方法 7日龄SD大鼠96只,随机分为假手术组、模型组、针灸组、超声组,采用经典Rice法制成HIBD模型。术后7、14、21d分别用悬吊实验检测大鼠前肢肌力,免疫组织化学染色和RT-PCR方法检测大鼠海马生长相关蛋白-43(growth associatedprotein 43,GAP-43)蛋白及mRNA的表达。结果超声组肌力明显优于模型组,GAP-43蛋白及mRNA的表达超声组高于模型组,且超声组和针灸组无明显差异。结论聚焦超声穴位治疗可上调GAP-43的表达,促进HIBD大鼠神经功能的康复。Objective To expore the effects of focused ultrasound irradiation on the baihui acupoint at neurorehabilitation of hypoxic ischemic brain damaged （HIBD） in neonatal rats. Methods 96 SD rats（7 days old） were randomly divided into sham operation group,model group ,acupuncture group and ultrasound group. The HIBD models were induced by using Rice＇S. After operation 7,14,21 days, the forelimb muscle strength was evaluated by suspension method. The expression of GAP-43 in the hippocampal were detected by immunohistochemical and RT-PCR method. Results The muscle strength and the expression of GAP-43 in hippocampus of ultrasound group were significant increased, as compared with model group ,no significant difference was found between the ultrasound group and the acupuncture group. Conclusions Focused ultrasound irradiation to the Bai Hui acupoint in HIBD rats can up-regulate the expression of GAP-43,improve the forelimb muscle strength.

关 键 词：聚焦超声 缺血缺氧性脑损伤 生长相关蛋白-43 

分 类 号：R445.1[医药卫生—影像医学与核医学] R245[医药卫生—诊断学]