用于骨力生物学研究的加载装置  

A loading device for study of bone mechanobiology

作　　者：徐强[1] 张春秋[1] 郭勇[2] 武汉[3] 董心[4] 

机构地区：[1]天津理工大学机械工程学院,天津市300384 [2]军事医学科学院卫生装备研究所,天津市300161 [3]吉林大学中日联谊医院,吉林省长春市130022 [4]吉林大学材料与工程学院,吉林省长春市130025

出　　处：《中国组织工程研究与临床康复》2011年第24期4379-4382,共4页Journal of Clinical Rehabilitative Tissue Engineering Research

基　　金：国家自然科学基金资助项目(10832012)"骨重建的生物力学机理及其在骨相关疾病诊治中的应用";(11072087)"双频加载对骨力生物学影响的相关研究"和(30772209)"体外骨组织工程化培养中组织空心化现象机理的研究"的部分资助;吉林省自然基金(201115023)"仿生条件下骨组织工程化培养力学响应的研究"的部分资助~~

摘　　要：背景:关节软骨组织细胞对力学刺激产生反馈来维持它的形态和结构,进而适应环境。加载装置能提供研究关节软骨力生物学的合适的力学环境。目的:根据组织工程仿生的原理,建立一种用于软骨力学生物学研究的双频加载装置。方法:该力学环境采用双频加载方式实现,通过控制可调凸轮和压电陶瓷振幅和频率来实现低频-高幅载荷耦合高频-低幅载荷,同时使用有限元法对受载体进行力学分析。结果与结论:按照仿真的原理,研制出用于软骨力生物学研究的加载装置。在双频加载的条件下,软骨浅表层受到的力学载荷最大,其次是中间层,深层受到的力学应力最小;在高频10Hz和20Hz与低频1Hz和2Hz叠加时,软骨表现出不同的力学响应,但是二者引起的差异很小。该力学环境的构建可能有助于组织工程的发展和临床医学的应用,未来将采用生物学实验进行检验。BACKGROUND：Articular cartilage cells respond to mechanical stimulation to maintain the morphological structure near cartilage of human body.A loading device can provide mechanical environment for studying mechanobiology.OBJECTIVE：To construct a dual-frequency loading device according to the bionic principle of tissue engineering.METHODS：This mechanical environment was achieved by using dual-frequency loading method and implementing the coupling of low-frequency high-amplitude mechanical loading and high-frequency low-amplitude mechanical loading by controlling amplitude and frequency of cam and piezoelectric ceramics respectively,meanwhile,finite element method was used to make mechanics analysis on cartilage.RESULTS AND CONCLUSION：In the light of the principle of simulation,a dual-frequency loading device has been developed for bone mechanobiology study.Under dual-frequency loading condition,mechanical loading on the surface layer of cartilage was largest,followed by the middle layer,smallest on the deep layer;when high-frequency of 10 and 20 Hz superpositioning low-frequency of 1 and 2 Hz,the cartilage show different mechanical responses;but the difference between two response was little.The construction of the mechanical environment of the device may be helpful to tissue engineering development and clinical applications;in addition,it will be tested with biological experiment in future.

关 键 词：双频加载装置 组织工程 关节软骨 有限元 组织构建 

分 类 号：R318[医药卫生—生物医学工程]