磷酸化p38丝裂原活化蛋白激酶与转化生长因子β1在正常和腰椎间盘退变组织中的表达  

Phosphorylated p38 mitogen-activated protein kinase and transforming growth factor beta-1 expression in degenerative versus normal disc tissues

作　　者：原野[1] 赵静[2] 赵杰[2] 李永民[1] 王旭[1] 

机构地区：[1]唐山市第二医院脊柱外科,河北省唐山市063000 [2]河北联合大学病理教研室,河北省唐山市063000

出　　处：《中国组织工程研究与临床康复》2011年第24期4423-4426,共4页Journal of Clinical Rehabilitative Tissue Engineering Research

基　　金：河北省教育厅自然科学指导计划项目(Z2005102);课题名称为"TGF-1;MMPs-1;MMPs-3在退变椎间盘组织中的表达及相关性研究";河北省科学技术研究与发展指导项目(052761422)资助;课题名称为"bFGF/TGF;MMPs/TIMPs在退变椎间盘组织中的表达研究"~~

摘　　要：背景:研究表明p38丝裂原活化蛋白激酶信号转导通路与椎间盘退变过程中炎症反应密切相关。目的:检测腰退变椎间盘组织和正常腰椎间盘组织中磷酸化p38丝裂原活化蛋白激酶和转化生长因子β1的表达。方法:选取经手术切除的36例退变椎间盘组织和10例正常椎间盘组织为对象,应用免疫组织化学法检测两组中磷酸化p38丝裂原活化蛋白激酶和转化生长因子β1的表达,并应用真彩色病理图像分析系统进行分析。结果与结论:腰退变椎间盘组织中磷酸化p38丝裂原活化蛋白激酶和转化生长因子β1的阳性表达率均高于正常椎间盘组织(P<0.05),证实磷酸化p38丝裂原活化蛋白激酶和转化生长因子β1在退变椎间盘组织中呈高表达。BACKGROUND：Studies show that p38 mitogen activated protein kinase（MAPK） signal pathway is highly correlated with inflammatory reactions during intervertebral disc degeneration.OBJECTIVE：To study the phosphorylated p38MAPK（p-p38MAPK） and transforming growth factor β1（TGF-β1） expression in patients with intervertebral disc degeneration.METHODS：Degenerative disc tissues resected by surgery from 36 patients with lumbar disc herniation were selected and normal disc tissues from 10 cases of trauma were used as control group.The expression of p-p38MAPK and TGF-β1 was evaluated with immunohistochemistry method and analyzed using pathological imaging analysis system.RESULTS AND CONCLUSION：The positive rate of p-p38MAPK and TGF-β1 expression in degenerative disc tissues was greater than normal disc tissue（P 0.05）.Results demonstrated that p-p38MAPK and TGF-β1 are highly expressed in the process of disc degeneration.

关 键 词：磷酸化P38丝裂原活化蛋白激酶 腰退变椎间盘组织 转化生长因子Β1 免疫组织化学 骨组织工程 

分 类 号：R318[医药卫生—生物医学工程]