人参皂苷Rd干预体外培养肝储脂细胞胶原的表达  

Effect of ginsenoside Rd on collagen expression in liver fat-storing cells

作　　者：谯时文[1] 王继丰[2] 黄勤[1] 朱宗贵[1] 张知贵[1] 

机构地区：[1]乐山职业技术学院护理系解剖教研室,四川省乐山市614000 [2]泸州医学院解剖学教研室,四川省泸州市646000

出　　处：《中国组织工程研究与临床康复》2011年第24期4457-4460,共4页Journal of Clinical Rehabilitative Tissue Engineering Research

摘　　要：背景:肝储脂细胞产生胶原是导致肝硬化的直接原因,调控肝储脂细胞产生胶原的能力即可以防治肝硬化。目的:观察人参皂苷Rd对体外培养肝储脂细胞Ⅰ,Ⅲ型胶原表达的影响。方法:体外培养肝储脂细胞,随机分为对照组和实验组,实验组用100mg/L人参皂苷Rd进行培养干扰,用免疫组织化学、免疫荧光技术检测Ⅰ,Ⅲ型胶原基因的表达。结果与结论:实验组比对照组,Ⅰ,Ⅲ型胶原阳性产物吸光度和面积比值明显降低(P<0.05),说明人参皂苷Rd对体外培养肝储脂细胞Ⅰ,Ⅲ型胶原的表达有明显调节作用,影响肝储脂细胞产生胶原纤维。BACKGROUND：Collagens produced by liver fat-storing cells are the directly reasons for cirrhosis,thus,regulating capacity of fat-storing cells producing collagens can prevent cirrhosis.OBJECTIVE：To explore effect of ginsenoside Rd on expression of type Ⅰ and Ⅲ collagens in liver fat-storing cells in vitro.METHODS：The liver fat-storing cells were cultured in vitro and interfered with ginsenoside Rd.The expression of type Ⅰ and Ⅲ collagens was detected by immunohistochemistry and immunofluorescent.RESULTS AND CONCLUSION：Compared with the control group,the positive/negative-area and the absorbance of the experimental group were obviously decreased（P 0.05）,suggesting that ginsenoside Rd regulates the expression of type Ⅰ and Ⅲ collagens in liver fat-storing cells in vitro,which affects liver fat-storing cells producing collagens.

关 键 词：人参皂苷 肝 储脂细胞 胶原 免疫组织化学 免疫荧光 

分 类 号：R318[医药卫生—生物医学工程]