一种免疫荧光图像减影技术在鉴定drebrin E亚型的应用  

Application of an immunofluorescence image subtraction technique in identification of drebrin isoform E

作　　者：宋明桥[1] 迟晓冬[1] 郑文旭[1] 刘萍[1] 

机构地区：[1]大连市友谊医院神经内科,大连116001

出　　处：《神经解剖学杂志》2011年第4期443-446,共4页Chinese Journal of Neuroanatomy

摘　　要：目的:在缺乏drebrin E抗体的情况下利用一种新的免疫荧光计算机图像减影技术的方法鉴定drebrinE亚型。方法:应用drebrin非特异性抗体M2F6和drebrin A特异性抗体DAS2对成年大鼠脑冰冻切片进行双重免疫荧光染色,然后用计算机图像处理软件对同一切片的两种染色照片进行图像减影技术处理,观察drebrin E在成年大鼠脑中的分布情况。结果:通过免疫荧光计算机图像减影技术得到了drebrin E亚型的图像,确认了drebrin E在成年大鼠脑中吻侧迁移流、海马齿状回、梨状皮质的分布。结论:利用免疫荧光图像减影技术可以实现对drebrin E亚型的鉴定,为类似drebrin这样存在两种亚型的蛋白质鉴定提供了一种新的方法。Objective： To identify the drebrin isoform E by a novel immunofluorescence image subtraction technique without drebrin E-specific antibody.Methods： We used drebrin-non-specific antibody M2F6 and drebrin A-specific antibody DAS2 to immunostain the frozen sections of the adult rat brain by double immunofluorescence staining.Then the two immunostaining images of the same section were processed with the image subtraction technique using a computer image processing software,and the expression of drebrin E in the adult rat brain was observed.Results： We obtained the images of drebrin isoform E by the immunofluorescence image subtraction technique.The expression of drebrin E in the rostral migratory stream,the dentate gyrus of the hippocampus and the piriform cortex of the adult rat brain was identified.Conclusion： The immunofluorescence image subtraction technique can identify the drebrin isoform E,and offer a novel approach to identify the proteins which have two isoforms similar to drebrin.

关 键 词：免疫荧光染色 计算机图像减影技术 DREBRIN E 成年神经元再生 

分 类 号：R392.1[医药卫生—免疫学]