Effects of low potassium dextran glucose solution on oleic acid-induced acute lung injury in juvenile piglets  

作　　者：LING Feng LIU Ying-long LIU Ai-jun WANG Dong WANG Qiang 

机构地区：[1]Department of Pediatric Cardiac Surgery Center, Fuwai Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100037, China

出　　处：《Chinese Medical Journal》2011年第14期2196-2202,共7页中华医学杂志（英文版）

基　　金：This study was supported by grants from the National Natural Science Foundation of China （No. 81070055） and the Beijing City Education Committee （No. PXM2011_014226 _07_000060）

摘　　要：Background Epithelial dysfunction in lungs plays a key role in the pathogenesis of acute lung injury. The beneficial effects of low potassium dextran glucose solution （LPD） have been reported in lung preservation, and LPD enables injured alveolar pneumocytes to recover. So we hypothesized that systemic administration of LPD may have benefits in treating acute lung injury. We investigated the effects of LPD on arterial blood gas and levels of some cytokines in oleic acid-induced acute lung injury in juvenile piglets.Methods Oleic acid （0.1 ml/kg） was intrapulmonarily administered to healthy anesthetized juvenile piglets. Ten animals were randomly assigned to two groups （n=5 each）： oleic acid-induced group （control group） with intravenous infusion of 12.5 ml/kg of lactated Ringer＇s solution 30 minutes before administration of oleic acid and LPD group with systemic administration of LPD （12.5 ml/kg） 30 minutes before injecting oleic acid. Blood gas variables and concentrations of tumor necrosis factor alpha, endothelin 1 and interleukin 10 were measured before and every 1 hour for 6 hours after initial lung injury.Results Compared with control group, blood pH, partial pressure of arterial oxygen to fraction of inspired oxygen ratio,partial pressure of arterial carbon dioxide, and mean pulmonary arterial pressure in LPD group were improved （P＜0.05or 0.01）. Six hours after lung injury, concentration of tumor necrosis factor alpha in lung tissue was lower in LPD group than control group （P＜0.05）. Plasmic concentration of endothelin 1 showed lower in LPD group while plasmic concentration of interleukin 10 showed higher in LPD group （P＜0.05）.Conclusions Before lung injury, systemic administration of LPD can improve gas exchange, attenuate pulmonary hypertension, decrease plasmic levels of endothelin 1, increase interleukin 10 and decrease concentration of tumor necrosis factor alpha in lung tissue in oleic acid-induced acute lung injury in juvenile piglets.Background Epithelial dysfunction in lungs plays a key role in the pathogenesis of acute lung injury. The beneficial effects of low potassium dextran glucose solution （LPD） have been reported in lung preservation, and LPD enables injured alveolar pneumocytes to recover. So we hypothesized that systemic administration of LPD may have benefits in treating acute lung injury. We investigated the effects of LPD on arterial blood gas and levels of some cytokines in oleic acid-induced acute lung injury in juvenile piglets.Methods Oleic acid （0.1 ml/kg） was intrapulmonarily administered to healthy anesthetized juvenile piglets. Ten animals were randomly assigned to two groups （n=5 each）： oleic acid-induced group （control group） with intravenous infusion of 12.5 ml/kg of lactated Ringer＇s solution 30 minutes before administration of oleic acid and LPD group with systemic administration of LPD （12.5 ml/kg） 30 minutes before injecting oleic acid. Blood gas variables and concentrations of tumor necrosis factor alpha, endothelin 1 and interleukin 10 were measured before and every 1 hour for 6 hours after initial lung injury.Results Compared with control group, blood pH, partial pressure of arterial oxygen to fraction of inspired oxygen ratio,partial pressure of arterial carbon dioxide, and mean pulmonary arterial pressure in LPD group were improved （P＜0.05or 0.01）. Six hours after lung injury, concentration of tumor necrosis factor alpha in lung tissue was lower in LPD group than control group （P＜0.05）. Plasmic concentration of endothelin 1 showed lower in LPD group while plasmic concentration of interleukin 10 showed higher in LPD group （P＜0.05）.Conclusions Before lung injury, systemic administration of LPD can improve gas exchange, attenuate pulmonary hypertension, decrease plasmic levels of endothelin 1, increase interleukin 10 and decrease concentration of tumor necrosis factor alpha in lung tissue in oleic acid-induced acute lung injury in juvenile piglets.

关 键 词：acute lung injury low potassium dextran cytokine PIGLET oleic acid 

分 类 号：S858.28[农业科学—临床兽医学] TQ226.36[农业科学—兽医学]