生脉注射液联合血塞通注射液对心肌梗死大鼠室颤阈值和心肌连接蛋白43表达的影响  

Effects of Chinese herbal medicines Shengmai injection and Xuesaitong injection on ventricular fibrillation threshold and connexin 43 expression in rats with myocardial infarction

作　　者：吴爱明[1,2,3] 张冬梅[1,2,3] 娄利霞[1,2,3] 翟建英 吕晞滢[1,2,3] 柴立民[1,2,3] 王硕仁[1,2,3] 

机构地区：[1]北京中医药大学东直门医院中医内科学教育部和北京市重点实验室,北京100700 [2]北京中医药大学东直门医院 [3]国家中医药管理局心脉病证益气活血重点研究室,北京100700 [4]中药复方新药开发国家工程中心,北京100079

出　　处：《中西医结合学报》2011年第7期775-782,共8页Journal of Chinese Integrative Medicine

基　　金：北京中医药大学自主选题资助项目(No.2009JYBZZ-JS079)

摘　　要：目的:探讨益气活血中药(生脉注射液联合血塞通注射液)对心肌梗死(myocardial infarction,MI)大鼠室颤阈值、心脏大小、心肌梗死百分比及心肌缝隙连接蛋白43(connexin43,Cx43)表达的影响。方法:将100只雄性SD大鼠随机分为假手术组、模型组、益气活血组和卡托普利组,采用结扎冠状动脉前降支的方法建立MI模型,假手术组只穿线,不结扎。于造模成功后第2天开始给予相应药物治疗,疗程为1个月。治疗结束后,采用在体心脏电刺激法检测心脏室颤阈值,称取全心质量后计算心脏质量指数(全心质量/体质量),图像分析测量左室内径和心肌梗死百分比,实时荧光定量聚合酶链式反应检测心肌Cx43mRNA的表达,免疫组织化学法检测心肌Cx43蛋白的表达。结果:与假手术组比较,模型组大鼠室颤阈值显著降低,心脏质量指数和左室内径明显增大,心肌Cx43mRNA及蛋白表达均明显降低(P<0.01)。与模型组比较,益气活血组和卡托普利组室颤阈值显著提高,心脏质量指数、左室内径和心肌梗死百分比均明显减小(P<0.05或P<0.01)。益气活血组Cx43mRNA和蛋白的表达水平均显著提高(P<0.05或P<0.01),而卡托普利组仅Cx43蛋白表达的光密度均值和综合光密度均值有明显的提高(P<0.05)。益气活血中药提高MI大鼠心肌Cx43mRNA和蛋白表达的作用优于卡托普利(P<0.05)。结论:生脉注射液联合血塞通注射液能提高MI大鼠心脏室颤阈值,其机制与改善MI后心脏结构重构和减轻心肌Cx43的表达有关。Objective：To explore the effects of Shengmai injection and Xuesaitong injection,compound Chinese herbal medicines for replenishing qi and activating blood,on ventricular fibrillation threshold,heart structure and connexin 43（Cx43）expression in rats with myocardial infarction（MI）.Methods：One hundred male SD rats were randomly divided into sham operation group,model group,Yiqi Huoxue（YQHX）group（Shengmai injection plus Xuesaitong injection）and captopril group.MI model of rats was established by ligating left anterior descending coronary artery,and rats in sham operation group were prepared in the same way except for the ligation of coronary artery.Rats were treated with corresponding drugs for 1 month from next day after modeling.After treatment ventricular fibrillation threshold was detected,and heart weight index,left ventricular internal diameter and percentage of myocardial infarction were measured.Expression of Cx43 mRNA in myocardium was detected by real-time fluorescent quantitative polymerase chain reaction,and expression of Cx43 protein was observed by immunohistochemical method.Results：Compared with the sham operation group,ventricular fibrillation threshold decreased significantly,heart weight index and left ventricular internal diameter increased,while expressions of Cx43 mRNA and protein decreased remarkably in the model group（P0.01）.Compared with the model group,ventricular fibrillation threshold was increased significantly,heart weight index,left ventricular internal diameter and percentage of myocardial infarction were decreased significantly in the YQHX group and captopril group（P0.05 or P0.01）.When it comes to expression of Cx43,both Cx43 mRNA and protein expressions were increased remarkably in the YQHX group compared with the model group（P0.05 or P0.01）,while only density mean and integral optical density of Cx43 protein expression were increased significantly in the captopril group（P0.05）.The enhancements on Cx43 mRNA and positive area sum of Cx43 protein in

关 键 词：生脉注射液 血塞通注射液 心肌梗死 室颤阈值 连接蛋白43 心室重构 大鼠 

分 类 号：R285.5[医药卫生—中药学]