老年患者恶性胸水中肿瘤浸润免疫细胞体外活化  

Research on activating tumor infiltrating immunocytes from malignant pleural effusion in old patient in vitro

作　　者：黄艳[1] 王红阳[1] 郭继芳[1] 刘飒[1] 

机构地区：[1]华北煤炭医学院附属医院呼吸内科,河北唐山063000

出　　处：《中国老年学杂志》2011年第14期2612-2614,共3页Chinese Journal of Gerontology

基　　金：河北省卫生厅重点课题(04258)

摘　　要：目的观察老年患者恶性胸水中肿瘤浸润免疫细胞的活性。方法分离恶性胸水单个核细胞(PEMCs),采用两步贴壁法,获得非贴壁细胞,树突细胞及淋巴细胞是其主要功能细胞成分。IL-2活化肿瘤浸润免疫细胞,SP法检测T淋巴细胞亚群的数量及免疫功能,SP法S-100蛋白染色检测树突细胞。结果 IL-2活化培养7 d后肿瘤浸润树突细胞(TIDC)和肿瘤浸润T淋巴细胞(TIL)数量明显增加(P<0.01)。恶性胸水TIDC经过IL-2活化后具有抗原提呈功能。结论 IL-2活化肿瘤微环境中TIDC,使其恢复免疫监视功能,有效地负载肿瘤抗原,协同TIL等其他免疫细胞有效杀伤肿瘤细胞。Objective To observe the activity of tumor infiltrating immunocytes in malignant pleural effusions from old patients.Methods Pleural effusions mononuclear cells（PEMCs） were separated and obtained.IL-2 was used to activate tumor infiltrating immunocytes,SP method was used to detect the number and function of T lymphocytes and S-100 protein was used to examine dendritic cells.Results After activated by IL-2 for 7 days,the number of tumor infiltrating dendritic cells（TIDC） and tumor infiltrating T lymphocytes（TIL） were increased（P〈0.01）.Activated TIDC had antigen presentation function.Conclusions IL-2 can activate TIDC in tumor microenvironment and recover its immune surveillance function and make it effectively to load tumor antigen and kill tumor cells cooperating with TIL and other immunocytes.

关 键 词：IL-2 肿瘤浸润树突状细胞 肿瘤浸润性淋巴细胞 恶性胸水 

分 类 号：Q291[生物学—细胞生物学]