Characterization and fine mapping of an early senescence mutant (es-t) in Oryza sativa L.  

作　　者：YANG YaoLong RAO YuChun LIU HuiJuan FANG YunXia DONG GuoJun HUANG LiChao LENG YuJia GUO LonBiao ZHANG GuangHeng HU Jiang GAO ZhenYu QIAN Qian ZENG DaLi 

机构地区：[1]State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou 310006, China [2]College of Life and Environment Sciences, Hangzhou Normal University, Hangzhou 310036, China

出　　处：《Chinese Science Bulletin》2011年第23期2437-2443,共7页

基　　金：supported by the National Special Program for Research and Transgenic Plants (2011ZX08009-003);the National Key Basic Research Program of China (2007CB10920203);the National Natural Science Foundation of China (30971760)

摘　　要：An es-t (early senescence-temporary) mutant, produced by ethylene methylsulfonate treatment of strain Nipponbare, was identified in rice. The leaves of es-t appeared yellow at the seedling stage, and had decreased chlorophyll content. Rust spots were found during growth in es-t, especially at the leaf margin and tip. The plants showed a typical early-senescence phenotype at the milky stage. The leaf surface of es-t appeared smoother than wild-type leaves under a scanning electron microscope, because the leaves lack siliceous protuberances around the stoma. Chloroplasts grow abnormally and are filled with many starch grains in es-t. Paraffin section analysis showed that the development of the sclerenchyma cells and vascular bundles were also abnormal in es-t. Genetic analysis indicated that es-t was controlled by a recessive gene, which was finely mapped to a 42-kb interval on chromosome 5. These results will facilitate the positional cloning and functional studies of the gene.An es-t （early senescence-temporary） mutant, produced by ethylene methylsulfonate treatment of strain Nipponbare, was identi- fied in rice. The leaves of es-t appeared yellow at the seedling stage, and had decreased chlorophyll content. Rust spots were found during growth in es-t, especially at the leaf margin and tip. The plants showed a typical early-senescence phenotype at the milky stage. The leaf surface of es-t appeared smoother than wild-type leaves under a scanning electron microscope, because the leaves lack siliceous protuberances around the stoma. Chloroplasts grow abnormally and are filled with many starch grains in es-t. Paraffm section analysis showed that the development of the sclerenchyma cells and vascular bundles were also abnormal in es-t. Genetic analysis indicated that es-t was controlled by a recessive gene, which was finely mapped to a 42-kb interval on chromo- some 5. These results will facilitate the positional cloning and functional studies of the gene.

关 键 词：ES 衰老 早期 精细定位 突变 水稻 扫描电子显微镜 表征 

分 类 号：S511[农业科学—作物学]