异氟醚和七氟醚对新生大鼠脑皮质Caspase-3及微管相关蛋白1B表达的影响  

Different effects of isoflurane and sevoflurane on expression of Caspase-3 and microtubule associated protein 1B of cortical neurons in neonatal SD rats

作　　者：张静[1] 李玉娟[1] 付艳妮[1] 曾敏婷[1] 陈伟强[1] 

机构地区：[1]中山大学附属孙逸仙纪念医院麻醉科,广州510120

出　　处：《中华行为医学与脑科学杂志》2011年第7期617-620,共4页Chinese Journal of Behavioral Medicine and Brain Science

基　　金：国家自然科学基金项目（30700787/C03030301）

摘　　要：目的研究同等剂量的异氟醚和七氟醚对新生SD大鼠第一躯体感觉皮质（Primary Somatosensory cortex，SI）Caspase-3表达以及皮质微管相关蛋白1B（microtubule associated protein 1B，MAP1B）表达的不同影响。方法出生后7d（P7）的新生SD大鼠5窝，每窝取11只，随机均分为异氟醚组（Ⅰ组），七氟醚组（S组）和对照组（C组）。各组分别吸人1．1％异氟醚或1．8％七氟醚或空气4h，在麻醉结束后2h每窝各取1只幼鼠灌注取脑，免疫组织化学法检测皮质SI区Caspase-3表达；另外，C组在麻醉处理0h，Ⅰ组和S组分别在麻醉2h，4h每窝各取1只幼鼠取新鲜脑皮质，Western Blot检测MAP1B表达量的变化。结果Ⅰ组和SI区Caspase-3表达分别较对照组增加561．23％（t=4．45，P〈0．01）和194．46％（t=5．17，P〈0．001），Ⅰ组比S组增加124．45％（P〈0．05）；Ⅰ组皮质MAP1B在麻醉2h和4h分别较对照组增加了557．15％（t=16．54P〈0．01）和475．21％（t=32．97，P〈0．01），MAP1B表达量2h与4h相比无统计学差异（P〉0．05）；S组皮质MAP1B在麻醉2h和4h分别较对照组增加了693．11％（t=9．45，P〈0．01）和268．15％（t=2．79，P=0．049），4h组表达量较2h降低了53．65％（P〈0．01）。结论0．5MAC异氟醚比七氟醚诱导更多新生大鼠大脑皮质神经细胞表达Caspase-3，两者均可诱导幼鼠上调大脑皮质MAP1B的表达来启动机体自我修复功能。Objective To investigate the effects of isoflurane and sevoflurane at the same dose on expression of Caspase-3 of primary somatosensory cortex （ SI ） and expression of microtubule associated protein 1B （ MAP1 B） in cortical neurons in neonatal SD rats. Methods Fifty-five neonatal SD rats at postnatal day 7 （ eleven rats each litter,altogether 5 litters） were assigned randomly into control group （C group）, isoflurane group （I group） and sevoflurane group （S group） in average. The rats in I group, S group or C group were exposed to 1.1% isoflurane or 1.8% sevoflurane （equivalent to 0.5MAC） or air 4h. The brain of neonatal rats were perfused and embedded by paraffin, Caspase-3 positive expression in the SI cortex of brain was detected by immunohistochemistry staining. Besides,the fresh cortex was dissected at 0 h in C group and at 2 h,4 h in I group and S group, microtubule associated protein 1B expression was detected by West blot staining. Results Caspase-3 positive cells in the SI cortex were increased by 561.23% in I group （ t=4.45, P〈0.01 ）and 194.46% in S group（ t = 5.17, P 〈 0.01 ） when compared with C group, and increased by 124.45% in I group （P 〈 0.05 ） when compared with S group. The MAP1B protein was increased by 557.15 % at 2 h （ t = 16.54 P 〈 0.01 ）and 475.21% at 4h（ t =32.97, P〈0.01）in I group while increased by 693.11% at 2h（ t=9.45, P〈0.001）and 268.15% at4 h（ t= 2.79, P = 0. 049）in S group when compared with C group. In S group, MAP1B protein at 4 h reduced by 53.65% （P〈 0.01 ） when compared with that at 2 h. Conclusion 0.5 MAC isoflurane can induce more apoptosis in the cortex in the neonatal rats＇brain at postnatal day 7 than sevoflurane. They can both significantly promote the expression of MAP1B in the cortex to start the self-reparation.

关 键 词：异氟醚 七氟醚 脑 CASPASE-3 微管相关蛋白1B 

分 类 号：R614[医药卫生—麻醉学]