基于SPR干涉成像的生物分子相互作用检测方法  

Biomolecular interaction detection based on SPR interferometric imaging

作　　者：王大千[1] 丁丽丽[1] 张玮[1] 邓焱[1] 章恩耀[1] 余兴龙[1] 

机构地区：[1]清华大学精密仪器与机械学系精密测试技术及仪器国家重点实验室,北京100084

出　　处：《清华大学学报（自然科学版）》2011年第5期612-616,共5页Journal of Tsinghua University(Science and Technology)

基　　金：国家自然科学基金资助项目(30727001)

摘　　要：蛋白质组学研究和药物开发迫切需要高通量、高精度、无标记且实时检测技术。该文提出了一种基于干涉成像的表面等离子共振(SPR)生物分子相互作用检测方法,在入射光的p和s偏振分量之间分别附加90°和-90°位相差,采集对应的干涉图像,并利用所采集的2帧图像,计算出反映传感面折射率分布的信号。循环采集能够实时获得生物反应进行时传感表面发生的折射率分布变化。这种方法在克服光源非均匀性影响的同时,还抑制了光强漂移引起的测量误差,达到了高精度。实验结果表明:干涉成像的图像一致性好,系统的折射率分辨率达到了2×10-6RIU(refractive index unit),动态检测区间大于0.005 RIU。在此基础上,检测了兔IgG和羊抗兔IgG的相互作用,结果表明表面等离子体共振干涉成像系统适于高通量检测,完善后有可能成为一种蛋白质组学研究和药物开发的强有力工具。Proteomics research and medical development require high-throughput,sensitive,label-free and real time detection techniques.A biomolecular interaction detection method was developed based on surface plasmon resonance（SPR） interferometric imaging.Phase differences of 90° or-90° were added between the p-and s-polarized components of the incident light to acquire two interferometric images.The signal distribution which gives the refractive index distribution can be calculated from the two interferometric images.The process is repeated to measure variations of the refractive index of biomolecular interaction sensing spots with time.This method is not affected by non-uniform light distributions and power drift and is very sensitive.Experiments demonstrate that the distributions calculated with this method are consistent with a refractive index resolution of about 2×10^-6 RIU（refractive index unit） and a dynamic range of over 0.005 RIU.Rabbit IgG and goat-anti-rabbit IgG interaction detection experiments demonstrate that this method is suitable for high-throughput detection.

关 键 词：生物分子相互作用检测 表面等离子体共振 高通量 干涉成像 

分 类 号：R197.39[医药卫生—卫生事业管理]