绵羊FecB基因打靶载体构建  

Construction of a new gene targeting vector in the sheep

作　　者：吴伟伟[1] 王力俭[1] 田可川[1] 徐新明[1] 张廷虎[1] 张艳花[1] 哈尼克孜[1] 

机构地区：[1]新疆畜牧科学院,新疆乌鲁木齐830000

出　　处：《畜牧与兽医》2011年第7期18-22,共5页Animal Husbandry & Veterinary Medicine

基　　金：国家绒毛用羊产业技术体系;自治区重大专项(200731132-7);农业科技成果转化资金项目(2008GB2G400352)

摘　　要：利用peGFP-C1,pRET.IL.PGK-TK和pMD19-T Simple等基本质粒,构建绵羊FecB基因打靶载体。以克隆载体pMD19-T Simple为载体骨架,插入一个正选择标记eGFP基因、一个负选择标记基因HSV-tk基因,并在eGFP基因两侧各添加一个同源长臂和同源短臂,从而构建绵羊FecB基因打靶载体。结果:经多个限制性内切核酸酶酶切鉴定和测序证实,所构建的基因打靶载体符合设计要求,表明成功构建了绵羊FecB基因打靶载体。In order to construct a sheep FecB gene targeting vector, plasmids of peGFP-C1, pRET. IL. PGK-TK and pMD19-T Simple and pMD19-T Simple cloning vector as the carrier frame with a positive selection marker eGFP gene and a negative selection marker gene HSV-tk gene were used. The correct structure of the targeting vector was confirmed by restriction enzyme digestion and sequencing analysis. Our experiment indicated that a FeeB gene targeting vector of sheep was successfully constructed.

关 键 词：FECB基因 基因打靶 载体构建 绵羊 

分 类 号：S826.8[农业科学—畜牧学]