前列康泰方对慢性前列腺炎前列腺组织TNF-α、IL-1βmRNA表达的影响  

Effects of Qian Lie Kang rai formula on the expressions of TNF-α、IL-1βmRNAin prostate tissues of rats with chronic prostatitis

作　　者：赵乔[1] 周安方[1] 曹继刚[1] 萧闵[1] 张晓艳[1] 

机构地区：[1]湖北中医药大学,武汉430065

出　　处：《中国男科学杂志》2011年第5期34-36,共3页Chinese Journal of Andrology

摘　　要：目的观察慢性细菌性和非细菌性前列腺炎大鼠前列腺组织中TNF-α、IL-1βmRNA的表达及前列康泰方对它们的影响。方法利用大鼠前列腺两侧叶注入预先稀释好的浓度为10^7～10^9个／ml大肠杆菌混悬液0．2ml来建立慢性细菌性前列腺炎病理模型；在大鼠前列腺侧叶注入25％消痔灵注射液0．2ml来建立慢性非细菌性前列腺炎病理模型。采用逆转录．聚合酶链反应（RT-PCR）的方法测定大鼠前列腺组织中TNF-α、IL-1βmRNA表达的变化，观察前列康泰方对它们的影响。结果与正常对照组比较，慢性细菌性和非细菌性前列腺炎模型组大鼠前列腺组织中TNF-α、IL-1βmRNA的表达均增强；与模型组比较，前列康泰方组大鼠前列腺组织中TNF-α、IL-1βmRNA的表达均降低。结论前列康泰方对慢性前列腺炎前列腺组织中细胞因子及其受体有一定的调控作用，这可能是该方治疗慢性前列腺炎的作用机制之一。Objective To analyze the changes of TNF-αandIL-1β expression in prostate tissues of rats with chronic bacterial and non-bacterial prostatitis treated by Qian Lie Kang Tai formula. Methods Rat model with chronic bacterial prostatitis was established by injecting with 0.2ml E.coli suspension with the concentration of 107-109/ml into two sides of prostate tissue. And rat model with chromc non-bacterial prostatitis was established by injecting with 0.2ml xiao zhi ling injection into two sides of prostate tissue. The levels TNF-α、IL-1βmRNA in rat prostate tissues were detected by RT-PCR. Resuluts Compared with that of the normal group, the expressions ofTNF-α、IL-1βmRNAin rat prostate tissue were all increased in model groups（P〈0.05）. Compared with other model groups, the expressions of TNF-α、IL-1β in prostate tissue were significantly decreased in Qian Lie Kang Tai formula group（P〈0.05）. Conclusion Qian Lie Kang Tai formula might regulate expressions of cytokines and their receptors in prostate tissue, which was the possible mechanism for treatment of chronic prostatitis.

关 键 词：前列康泰方 前列腺炎 肿瘤坏死因子-Α 白细胞介素1Β 

分 类 号：R697.33[医药卫生—泌尿科学]