凤冈野生天麻抗真菌肽基因(gaf)的克隆与序列分析  

Cloning and Sequence Analysis of Antifungal Peptide Gene(gaf) of Wild Gastrodia from Fenggang County in Guizhou Province

作　　者：何光志[1] 田维毅[1] 王平[1] 王文佳[1] 奚锦[1] 俞琦[1] 黄高[1] 蔡琨[1] 王乾宇[1] 刘安胜 安传伟[1] 查高武 张鹏 

机构地区：[1]贵阳中医学院,贵州贵阳550002 [2]凤冈天桥乡畜牧兽医水产站,贵州凤冈564217 [3]兴义乌沙镇格里小学,贵州兴义524005 [4]兴义捧乍中学,贵州兴义562407

出　　处：《贵州农业科学》2011年第7期28-30,33,共4页Guizhou Agricultural Sciences

基　　金：贵州省中药管理局课题"贵州野生天麻抗真菌肽基因的表达及重组蛋白抗真菌作用研究"

摘　　要：为了克隆凤冈野生天麻的抗真菌肽基因(gaf),以凤冈野生天麻的RNA为模板,采用RT-PCR技术扩增抗真菌肽基因(gaf),经克隆和测序结果显示:RT-PCR产物电泳扩增的目标条带长度为545 bp;经测序和序列分析,该基因与Genebank中公布的序列编码天麻抗真菌蛋白的基因序列(AY032588)同源性达97%。此研究成功地克隆了凤冈野生天麻抗真菌肽编码基因。The antifungal peptide gene（gaf） of wild Gastrodia from Fenggang county in Guizhou province was amplified by RT-PCR based on its RNA to clone its antifungal peptide gene（gaf）.The cloning and sequence analysis showed that the amplified gene was of 545bp,the homology between the antifungal peptide gene（gaf） of wild Gastrodia from Fenggang county and AY032588 listed in Genebank reached 97%,which indicates that the encoding gene of wild Gastrodia from Fenggang county in Guizhou is successfully cloned.

关 键 词：野生天麻 抗真菌肽基因(gaf) 克隆 序列分析 

分 类 号：S567.9[农业科学—中草药栽培]