高速逆流色谱制备性分离纯化白果内酯和橙皮甙对照品  

Preparative Separation and Purification of Authentic Pure Compounds of Bilobalide and Hesperidin by HSCCC

作　　者：蔡定国 佘佳红 刘文庸[2] 张兵 顾明娟 

机构地区：[1]海军药物研究中心,上海200081 [2]第二军医大学药学院,上海200433

出　　处：《中药新药与临床药理》1999年第6期364-366,共3页Traditional Chinese Drug Research and Clinical Pharmacology

摘　　要：首次应用ＨＳＣＣＣ 获得白果内酯和橙皮甙纯品。白果内酯经ＴＬＣ， ＨＰＬＣ／ＲＩ， ＭＳ （Ｅ１） 和ＩＲ 谱鉴定， 橙皮甙经ＨＰＬＣ／ ＵＶ 和ＴＬＣ 鉴定， 均达到定量用对照品的纯度和含量要求。表明ＨＳＣＣＣ 是制备性分离中草药对照品的一种简捷实用的新手段。As a liquid partition chromatographic system free of solid support matrix in the separation column, high-speed countercurrent chromatography (HSCC) was successfully applied to the separation of two authentic pure compounds for the first time.Bilobalide was isolated from Ginkgo Biloba Extract (GBE) and Hesperidin was purified from crude extraction of pericarp of Citrus reticulata Blanco, which can be used as reference standard of the analysis for Chinese herbal medicine.The results indicate that the HSCCC is an ideal technique for separating preparation of authentic pure compounds from medicinal plants.

关 键 词：液相色谱法 白果内酯 分离 提纯 橙皮甙 

分 类 号：R284.1[医药卫生—中药学]