成年大鼠伏隔核脑片神经元的细胞电生理特性  

Cellular electrophysiological characteristics of neurons in nucleus accumbens slices of adult rats

作　　者：顾强[1] 汪萌芽[1] 

机构地区：[1]皖南医学院细胞电生理研究室,安徽芜湖241002

出　　处：《皖南医学院学报》2011年第4期259-263,共5页Journal of Wannan Medical College

基　　金：安徽省自然科学基金项目(090413084)

摘　　要：目的:观察成年大鼠伏隔核(nucleus accumbens,NAc)脑片神经元的细胞电生理特性,为研究药物成瘾和神经精神性疾病提供一种离体伏隔核细胞模型。方法:对取自成年大鼠的伏隔核脑片神经元进行细胞内记录,并检测膜电学特性和局部电刺激诱发的突触反应。结果:测定记录稳定的10个NAc神经元的静息电位、膜斜率电阻和动作电位幅度分别为(-70.9±12.9)mV、(59.3±19.8)MΩ和(86.5±14.1)mV。在其中4个神经元观察到放电频率随刺激强度增大而升高,在6个NAc细胞观察到异常整流现象。对NAc神经元的背侧局部电刺激(0.1 Hz)可诱发刺激强度依赖性去极化突触反应(EPSP,n=5)。对5个NAc细胞灌流10 mmol/L的L-谷氨酸,产生去极化反应,而且灌流0.5μmol/L的河豚毒素可逆性取消动作电位和EPSP。结论:结果表明成年大鼠伏隔核脑片神经元细胞内记录技术稳定可靠,可用于相关脑功能和神经精神性疾病的细胞电生理和药理学分析。Objective：To observe the cellular electrophysiological characteristics of neurons in nucleus accumbens（NAc） slices of adult rats for providing an in vitro model of NAc to study drug addiction and neuropsychiatric disorders.Methods： The intracellular recordings were made in neurons of NAc slices prepared from adult rats,and observed were the membrane electrophysiological properties and synaptic transmission evoked by focal electrical stimulation.Results： Of the 10 NAc neurons stably recorded,the resting potential,slope membrane resistance and amplitude of action potential were（-70.9±12.9） mV,（59.3±19.8）MΩ and（86.5±14.1）mV,respectively,in which spike firing frequency was increased with depolarizing current intensity in 4 cells and anomalous rectification was found in 6 NAc neurons.The excitatory postsynaptic potentials（EPSPs） with stimulus intensity-dependent property were elicited by dorsal focal electrical stimuli at 0.1 Hz（n=5）.Superfusion of L-glutamate at concentration of 10 mmol/L induced depolarizing response in 5 NAc neurons tested.Still,bath of tetrodotoxin（0.5 μmol/L） reversibly nullified the action potential initiated by intracellular depolarizing current injection and EPSPs evoked by focal electrical stimulation.Conclusion： Intracellular recordings from NAc neurons of adult rats appear stable and reliable,suggesting that the typical electrophysiological properties and synaptic potentials may be used to test the physiological and pharmacological actions of NAc neurons associated with cerebral functions and neuropsychiatric disorders.

关 键 词：大鼠 伏隔核 细胞内记录 突触传递 电生理学 

分 类 号：R338.8[医药卫生—人体生理学]