胃促生长素对胃大部切除大鼠术后早期恢复的影响  

Effect of Ghrelin on Early Recovery of Rats after Subtotal Gastrectomy

作　　者：于嵩[1] 王志刚[1] 杨成广[1] 阎钧[1] 郑起[1] 

机构地区：[1]上海交通大学附属第六人民医院普外科,上海200233

出　　处：《中国普外基础与临床杂志》2011年第7期728-731,共4页Chinese Journal of Bases and Clinics In General Surgery

摘　　要：目的探讨应用外源性胃促生长素（ghrelin）对胃大部切除术后大鼠早期恢复的影响。方法将12只胃大部切除（毕Ⅰ式）大鼠随机均分为2组,分别于腹腔内注射生理盐水或胃促生长素,称量术前和术后1-7 d的大鼠体重和每日摄食量;术后第7天处死大鼠,实时荧光定量PCR法测定大鼠胃底组织中胃促生长素mRNA表达相对量,并检测吻合口爆破压和吻合口组织中羟脯氨酸含量。结果 2组大鼠之间术前及术后1-7 d体重的差异均无统计学意义（P〉0.05）。生理盐水组大鼠体重术后逐渐降低,并均明显低于术前（P〈0.01）,且在术后第5天达到最低（P〈0.01）,后逐渐增加,但仍均低于术前（P〈0.01）;胃促生长素组大鼠体重术后逐渐降低,除术后1 d与术前比较的差异无统计学意义（P=0.693）外,均明显低于术前（P〈0.01）,在术后第4天达到最低（P〈0.01）,后逐渐增加,但仍均低于术前（P〈0.05或P〈0.01）。胃促生长素组大鼠的累积摄食量为（52.50±6.77）g,明显高于生理盐水组大鼠的（45.67±7.47）g,P〈0.05。术后第7天胃促生长素组大鼠胃底组织中胃促生长素mRNA表达相对量为0.08±0.04,明显低于生理盐水组大鼠的0.22±0.07（P〈0.01）。胃促生长素组大鼠吻合口爆破压为（172.33±10.44）mm Hg（1 mm Hg=0.133 kPa）,明显高于生理盐水组的（155.83±6.62）mm Hg,P〈0.05。胃促生长素组大鼠吻合口组织中羟脯氨酸含量为（0.50±0.29）μg/mg wet tissue,明显高于生理盐水组大鼠的（0.43±0.05）μg/mg wet tissue,P〈0.01。结论 胃促生长素能有效促进胃大部切除术后大鼠的早期恢复,而外源性胃促生长素的摄入可抑制机体术后早期的负反馈性代偿作用。Objective To explore the effect of exogenous ghrelin on early recovery of rats after subtotal gastrectomy.Methods Twelve rats undergoing subtotal gastrectomy（B-Ⅰtype） were randomly divided into two groups,and saline or ghrelin was intraperitoneally injected in two groups,respectively.The body weight and daily food intake were measured before operation and on 1-7 d after operation.Rats were killed on day 7 after operation and the expressions of ghrelin mRNA in the fundus of stomach and anastomotic stoma was determined by real-time fluorescent quantitative PCR assay.The anastomotic bursting pressure and hydroxyproline content of anastomotic stoma tissues were also detected.Results There was no significant difference（P〈0.05） in pre and postoperative body weight between two groups.Gradual decrease in postoperative body weight among the rats of saline group was observed which was significantly lower than that before operation（P〈0.01）.Body weight reached it＇s lowest on day 1 after operation（P〈0.01）,after which it gradually increased but was still lower than that before operation（P〈0.01）.The postoperative body weight of rats in ghrelin group gradually decreased too,and was also significantly lower than preoperative body weight（P〈0.01）,except for the day 1 after operation（P=0.693）.It reached the lowest on day 4 after operation（P〈0.01）,then it gradually increased but was still lower than that before operation（P〈0.05 or P〈0.01）.The cumulative food intake of rats in ghrelin group was（52.50±6.77） g,which was significantly higher than that in saline group（（45.67±7.47） g）,P〈0.05.On day 7 after operation,relative expression of ghrelin mRNA in the fundus of stomach of rats in ghrelin group was 0.08±0.04,which was significantly lower than that in saline group（0.22±0.07）,P〈0.01.Compared with saline group,ghrelin-treated rats displayed significantly higher bursting pressure（（155.83±6.62） mm Hg vs.（172.33±10.44） mm Hg,P〈0.05） higher hydro

关 键 词：胃促生长素 胃大部切除 大鼠 吻合口 羟脯氨酸 

分 类 号：R656.61[医药卫生—外科学]