2010年婴幼儿腹泻轮状病毒监测分析  

Rotavirus surveillance among children with diarrhea in 2010

作　　者：杨学磊[1] 李玉静[2] 贾娜尔[1] 余亮[1] 

机构地区：[1]新疆维吾尔自治区人民医院,新疆乌鲁木齐830001 [2]石河子大学医学院

出　　处：《中国预防医学杂志》2011年第7期565-567,共3页Chinese Preventive Medicine

基　　金：国家自然基金(30760232);益普生腹泻研究基金(IDF-2007-12)

摘　　要：目的了解乌鲁木齐地区婴幼儿轮状病毒（RV）腹泻流行情况。方法 2010年1~12月,采集门诊或住院≤5岁婴幼儿腹泻样本503份,用胶体金法和ELISA检测RV抗原,阳性样本用逆转录-聚合酶链反应（RT-PCR）进行G、P基因型分型。结果 503份腹泻样本,RV阳性率为24.25%（122/503）。RV感染对象主要为35月以内婴幼儿（95.08%）。对102份RV阳性样本进行G/P分型,G3型是主要优势株,占46.08%,其次为G1型（34.31%）、G9型（11.76%）和G2型（2.94%）,混合G型感染占4.90%;P基因型以P[8]为主导,占67.65%,其次为P型混合感染（19.61%）、P[4]（6.86%）和P[6]（4.90%）,1例（0.98%）未能分型。最常见的G/P组合为G3P[8]（35.29%）和G1P[8]（27.45%）。结论 RV是乌鲁木齐地区婴幼儿腹泻的主要病原,2010年G3P[8]和G1P[8]为主要流行基因型。Objective To investigate the characteristics of rotavirus infection among infants and children with diarrhea in Urumqi.Methods A total of 503 fecal samples were collected from children under 5 years old with diarrhea and examined for rotavirus antigen by colloidal gold method and ELISA in 2010.Rotavirus strains were classified into G and P types with type-specific primers.Results Rotavirus was detected in 122 of 503（24.25%） diarrheal children.Ninety-five percent（95.08%） of patients with rotavirus diarrhea were less than 35 months old.G-typing showed that G3（44.08%） was predominant,followed by G1（34.31%）,G9（11.76%）,G2（4.94%） and G mixed infection（4.90%）.P[8] was predominant genotype（67.65%）,followed by P mixed（19.61%）,P[4]（6.86%）,P[6]（4.90%） and undetermined（1,1%） respectively for P-typing.The most common G/P combination were G3P[8]（33.29%） and G1P[8]（27.45%）.Conclusion Rotavirus was the major cause of diarrhea among young children in Urumqi.G3P[8] and G1P[8] strains were the most prevalent genotypes in 2010.

关 键 词：轮状病毒 腹泻 儿童 基因型 

分 类 号：R725.1[医药卫生—儿科]