16S rRNA基因序列分析法在输入性蜚蠊携带病原菌检测中的应用研究  

Application of 16S rRNA gene sequences on cockroach-carried pathogens detection

作　　者：朱临[1] 孙立新[2] 胡红霞[1] 叶松[1] 朱国强[2] 杨庆贵[2] 

机构地区：[1]安徽理工大学医学院,安徽淮南232001 [2]江苏出入境检验检疫局

出　　处：《中国国境卫生检疫杂志》2011年第3期158-161,共4页Chinese Journal of Frontier Health and Quarantine

基　　金：国家质检总局科研基金项目(2008IK261-02)

摘　　要：目的应用16SrRNA基因序列分析法对江苏口岸输入性蜚蠊携带的细菌进行快速检测。方法用研磨器将蜚蠊研碎,用LB培养基增菌和分离培养,随机挑选细菌单克隆,提取DNA,对16SrRNA基因进行PCR扩增,扩增产物进行序列分析、比对,确定细菌种属。结果从输入性蜚蠊中共分离鉴定出23种细菌,分属15属。结论输入性蜚蠊携带多种重要致病菌和条件致病菌,应加强口岸蜚蠊类监测和卫生处理,控制相应传染病的发生和流行。Objective To survey pathogens carried by imported cockroach at Jiangsu port with the analytical method of 16S rRNA gene sequences. Methods Smash the cockroach with a grinder, isolate the bacteria with LB, extract bacteria monoclonal DNA and then amplify 16S rRNA with PCR, and sequence analysis and identification bacteria species. Results In the imported Cockroaches, a total of 23 species bacteria was isolated and identified, belonging to 15 genera. Conclusion The results showed that imported cockroach carry a variety of pathogenic or opportunistic pathogens. it is needed to strengthen the monitoring on port cockroach, and prevent the occurrence and prevalence of the corresponding diseases.

关 键 词：输入性 蜚蠊 细菌 16S RRNA基因序列 检测 

分 类 号：R184.3[医药卫生—流行病学]